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Micromachines
Volume 13
Issue 12
10.3390/mi13122194
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Article

Rapid Detection of Lipopolysaccharide and Whole Cells of Francisella tularensis Based on Agglutination of Antibody-Coated Gold Nanoparticles and Colorimetric Registration

by
Nadezhda A. Byzova
1,
Anatoly V. Zherdev
1,*,
Alexey A. Gorbatov
2,
Anton G. Shevyakov
2,
Sergey F. Biketov
2 and
Boris B. Dzantiev
1
1
A.N. Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia
2
State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Russia
*
Author to whom correspondence should be addressed.
Micromachines 2022, 13(12), 2194; https://doi.org/10.3390/mi13122194
Submission received: 14 November 2022 / Revised: 7 December 2022 / Accepted: 8 December 2022 / Published: 11 December 2022
(This article belongs to the Special Issue Biosensors in Monitoring and Diagnosis for Medical and Agricultural Applications)
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Abstract

The paper presents development and characterization of a new bioanalytical test system for rapid detection of lipopolysaccharide (LPS) and whole cells of Francisella tularensis, a causative agent of tularemia, in water samples. Gold nanoparticles (AuNPs) coated by the obtained anti-LPS monoclonal antibodies were used for the assay. Their contact with antigen in tested samples leads to aggregation with a shift of absorption spectra from red to blue. Photometric measurements at 530 nm indicated the analyte presence. Three preparations of AuNPs with different diameters were compared, and the AuNPs having average diameter of 34 nm were found to be optimal. The assay is implemented in 20 min and is characterized by detection limits equal to 40 ng/mL for LPS and 3 × 104 CFU/mL for whole cells of F. tularensis. Thus, the proposed simple one-step assay integrates sensitivity comparable with other immunoassay of microorganisms and rapidity. Selectivity of the assay for different strains of F. tularensis was tested and the possibility to choose its variants with the use of different antibodies to distinguish virulent and non-virulent strains or to detect both kinds of F. tularensis was found. The test system has been successfully implemented to reveal the analyte in natural and tap water samples without the loss of sensitivity.
Keywords: immunoanalysis; agglutination; colloidal gold; tularemia; lipopolysaccharide; express diagnostics immunoanalysis; agglutination; colloidal gold; tularemia; lipopolysaccharide; express diagnostics

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MDPI and ACS Style

Byzova, N.A.; Zherdev, A.V.; Gorbatov, A.A.; Shevyakov, A.G.; Biketov, S.F.; Dzantiev, B.B. Rapid Detection of Lipopolysaccharide and Whole Cells of Francisella tularensis Based on Agglutination of Antibody-Coated Gold Nanoparticles and Colorimetric Registration. Micromachines 2022, 13, 2194. https://doi.org/10.3390/mi13122194

AMA Style

Byzova NA, Zherdev AV, Gorbatov AA, Shevyakov AG, Biketov SF, Dzantiev BB. Rapid Detection of Lipopolysaccharide and Whole Cells of Francisella tularensis Based on Agglutination of Antibody-Coated Gold Nanoparticles and Colorimetric Registration. Micromachines. 2022; 13(12):2194. https://doi.org/10.3390/mi13122194

Chicago/Turabian Style

Byzova, Nadezhda A., Anatoly V. Zherdev, Alexey A. Gorbatov, Anton G. Shevyakov, Sergey F. Biketov, and Boris B. Dzantiev. 2022. "Rapid Detection of Lipopolysaccharide and Whole Cells of Francisella tularensis Based on Agglutination of Antibody-Coated Gold Nanoparticles and Colorimetric Registration" Micromachines 13, no. 12: 2194. https://doi.org/10.3390/mi13122194

APA Style

Byzova, N. A., Zherdev, A. V., Gorbatov, A. A., Shevyakov, A. G., Biketov, S. F., & Dzantiev, B. B. (2022). Rapid Detection of Lipopolysaccharide and Whole Cells of Francisella tularensis Based on Agglutination of Antibody-Coated Gold Nanoparticles and Colorimetric Registration. Micromachines, 13(12), 2194. https://doi.org/10.3390/mi13122194

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