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Article

Fluorescence Anisotropy as a Temperature-Sensing Molecular Probe Using Fluorescein

1
Department of Mechanical Engineering, Faculty of Engineering, Tokyo University of Science, 6-3-1, Niijuku, Katsushika-ku, Tokyo 125-8585, Japan
2
Water Frontier Research Center, Research Institute for Science and Technology, Tokyo University of Science, 1-3, Kagurazaka, Shinjuku-ku, Tokyo 125-8585, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Sébastien Balme
Micromachines 2021, 12(9), 1109; https://doi.org/10.3390/mi12091109
Received: 29 July 2021 / Revised: 8 September 2021 / Accepted: 12 September 2021 / Published: 15 September 2021
Fluorescence anisotropy, a technique to study the folding state of proteins or affinity of ligands, is used in this present work as a temperature sensor, to measure the microfluidic temperature field, by adding fluorophore in the liquid. Fluorescein was used as a temperature-sensing probe, while glycerol–aq. ammonia solution was used as a working fluid. Fluorescence anisotropy of fluorescein was measured by varying various parameters. Apart from this, a comparison of fluorescence anisotropy and fluorescence intensity is also performed to demonstrate the validity of anisotropy to be applied in a microfluidic field with non-uniform liquid thickness. Viscosity dependence and temperature dependence on the anisotropy are also clarified; the results indicate an appropriate selection of relation between molecule size and viscosity is important to obtain a large temperature coefficient in anisotropy. Furthermore, a practical calibration procedure of the apparatus constant is proposed. In addition, the potential of temperature imaging is confirmed by the measurement of temperature distribution under focused laser heating. View Full-Text
Keywords: fluorescence anisotropy; fluorescein; microfluidics; fluorescence polarization; temperature imaging; thermal molecular probe fluorescence anisotropy; fluorescein; microfluidics; fluorescence polarization; temperature imaging; thermal molecular probe
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MDPI and ACS Style

Jain, P.; Aida, T.; Motosuke, M. Fluorescence Anisotropy as a Temperature-Sensing Molecular Probe Using Fluorescein. Micromachines 2021, 12, 1109. https://doi.org/10.3390/mi12091109

AMA Style

Jain P, Aida T, Motosuke M. Fluorescence Anisotropy as a Temperature-Sensing Molecular Probe Using Fluorescein. Micromachines. 2021; 12(9):1109. https://doi.org/10.3390/mi12091109

Chicago/Turabian Style

Jain, Puneet, Takuya Aida, and Masahiro Motosuke. 2021. "Fluorescence Anisotropy as a Temperature-Sensing Molecular Probe Using Fluorescein" Micromachines 12, no. 9: 1109. https://doi.org/10.3390/mi12091109

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