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Article

Loop-Mediated Isothermal Amplification in a Core-Shell Bead Assay for the Detection of Tyrosine Kinase AXL Overexpression

1
Queensland Micro- and Nanotechnology Centre, Griffith University, 170 Kessels Road, Nathan, QLD 4111, Australia
2
School of Environment and Science, Nathan Campus, Griffith University, 170 Kessels Road, Nathan, QLD 4111, Australia
*
Authors to whom correspondence should be addressed.
Both the authors contributed equally to the work.
Academic Editor: Francis Lin
Micromachines 2021, 12(8), 905; https://doi.org/10.3390/mi12080905
Received: 26 June 2021 / Revised: 22 July 2021 / Accepted: 27 July 2021 / Published: 30 July 2021
(This article belongs to the Special Issue Feature Papers of Micromachines in Biology and Biomedicine 2021)
The upregulated expression of tyrosine kinase AXL has been reported in several hematologic and solid human tumors, including gastric, breast, colorectal, prostate and ovarian cancers. Thus, AXL can potentially serve as a diagnostic and prognostic biomarker for various cancers. This paper reports the first ever loop-mediated isothermal amplification (LAMP) in a core-shell bead assay for the detection of AXL gene overexpression. We demonstrated simple instrumentation toward a point-of-care device to perform LAMP. This paper also reports the first ever use of core-shell beads as a microreactor to perform LAMP as an attempt to promote environmentally-friendly laboratory practices. View Full-Text
Keywords: loop-mediated isothermal amplification; AXL overexpression; core-shell bead assay loop-mediated isothermal amplification; AXL overexpression; core-shell bead assay
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MDPI and ACS Style

Sreejith, K.R.; Umer, M.; Singha, P.; Nguyen, N.-K.; Kasetsirikul, S.; Ooi, C.H.; Shiddiky, M.J.A.; Nguyen, N.-T. Loop-Mediated Isothermal Amplification in a Core-Shell Bead Assay for the Detection of Tyrosine Kinase AXL Overexpression. Micromachines 2021, 12, 905. https://doi.org/10.3390/mi12080905

AMA Style

Sreejith KR, Umer M, Singha P, Nguyen N-K, Kasetsirikul S, Ooi CH, Shiddiky MJA, Nguyen N-T. Loop-Mediated Isothermal Amplification in a Core-Shell Bead Assay for the Detection of Tyrosine Kinase AXL Overexpression. Micromachines. 2021; 12(8):905. https://doi.org/10.3390/mi12080905

Chicago/Turabian Style

Sreejith, Kamalalayam Rajan, Muhammad Umer, Pradip Singha, Nhat-Khuong Nguyen, Surasak Kasetsirikul, Chin Hong Ooi, Muhammad J. A. Shiddiky, and Nam-Trung Nguyen. 2021. "Loop-Mediated Isothermal Amplification in a Core-Shell Bead Assay for the Detection of Tyrosine Kinase AXL Overexpression" Micromachines 12, no. 8: 905. https://doi.org/10.3390/mi12080905

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