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Communication

An Effective SARS-CoV-2 Electrochemical Biosensor with Modifiable Dual Probes Using a Modified Screen-Printed Carbon Electrode

1
Department of General Medicine, Mater Misericordiae University Hospital, 351402 Dublin, Ireland
2
Department of Chemical Engineering and Biotechnology, Graduate Institute of Biochemical and Biomedical Engineering, National Taipei University of Technology, Taipei City 106, Taiwan
3
Division of Urology, Department of Surgery, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan
4
College of Medicine, Chang Gung University, Taoyuan 333, Taiwan
5
Department of Nursing, College of Medicine, Fu Jen Catholic University, New Taipei City 242, Taiwan
6
Division of Colorectal Surgery, Department of Surgery, Mackay Memorial Hospital, Taipei City 104, Taiwan
*
Authors to whom correspondence should be addressed.
Academic Editor: Aiqun Liu
Micromachines 2021, 12(10), 1171; https://doi.org/10.3390/mi12101171
Received: 31 August 2021 / Revised: 23 September 2021 / Accepted: 27 September 2021 / Published: 29 September 2021
(This article belongs to the Special Issue Advances in Biomedical Nanotechnology)
Due to the severe acute respiratory syndrome coronavirus (SARS-CoV-2, also called coronavirus disease 2019 (COVID-19)) pandemic starting in early 2020, all social activities ceased in order to combat its high transmission rate. Since vaccination combats one aspect for halting the spread of the virus, the biosensor community has looked at another aspect of reducing the burden of the COVID-19 pandemic on society by developing biosensors that incorporate point-of-care (POC) testing and the rapid identification of those affected in order to deploy appropriate measures. In this study, we aim first to propose a screen-printed carbon electrode (SPCE)-based electrochemical biosensor that meets the ASSURED criteria (i.e., affordable, sensitive, specific, user-friendly, rapid, equipment-free, and deliverable) for POC testing, but more importantly, we describe the novelty of our biosensor’s modifiability that uses custom dual probes made from target nucleic acid sequences. Additionally, regarding the sensitivity of the biosensor, the lowest sample concentration was 10 pM (p = 0.0257) without amplification, which might challenge the traditional technique of reverse transcriptase-polymerase chain reaction (RT-PCR). The purpose of this study is to develop a means of diagnostics for the current pandemic as well as to provide an established POC platform for future epidemics. View Full-Text
Keywords: SPCE biosensor; COVID-19; SARS-CoV-2 SPCE biosensor; COVID-19; SARS-CoV-2
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MDPI and ACS Style

Pang, S.-N.; Lin, Y.-L.; Yu, K.-J.; Chiou, Y.-E.; Leung, W.-H.; Weng, W.-H. An Effective SARS-CoV-2 Electrochemical Biosensor with Modifiable Dual Probes Using a Modified Screen-Printed Carbon Electrode. Micromachines 2021, 12, 1171. https://doi.org/10.3390/mi12101171

AMA Style

Pang S-N, Lin Y-L, Yu K-J, Chiou Y-E, Leung W-H, Weng W-H. An Effective SARS-CoV-2 Electrochemical Biosensor with Modifiable Dual Probes Using a Modified Screen-Printed Carbon Electrode. Micromachines. 2021; 12(10):1171. https://doi.org/10.3390/mi12101171

Chicago/Turabian Style

Pang, Sow-Neng, Yu-Lun Lin, Kai-Jie Yu, Yueh-Er Chiou, Wai-Hung Leung, and Wen-Hui Weng. 2021. "An Effective SARS-CoV-2 Electrochemical Biosensor with Modifiable Dual Probes Using a Modified Screen-Printed Carbon Electrode" Micromachines 12, no. 10: 1171. https://doi.org/10.3390/mi12101171

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