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Open AccessArticle

A Novel Electroporation System for Living Cell Staining and Membrane Dynamics Interrogation

by 1,†, 1,†, 1 and 2,*
1
HKU-Shenzhen Institute of Research and Innovation (HKU-SIRI), Guangdong 518057, China
2
Department of Mechanical Engineering, The University of Hong Kong, Hong Kong SAR, China
*
Author to whom correspondence should be addressed.
These two authors contributed equally.
Micromachines 2020, 11(8), 767; https://doi.org/10.3390/mi11080767
Received: 23 July 2020 / Revised: 7 August 2020 / Accepted: 10 August 2020 / Published: 11 August 2020
(This article belongs to the Special Issue Microfluidic Machines)
A novel electroporation system was developed to introduce transient membrane pores to cells in a spatially and temporally controlled manner, allowing us to achieve fast electrotransfection and live cell staining as well as to systematically interrogate the dynamics of the cell membrane. Specifically, using this platform, we showed that both reversible and irreversible electroporation could be induced in the cell population, with nano-sized membrane pores in the former case being able to self-reseal in ~10 min. In addition, green fluorescent protein(GFP)-vinculin plasmid and 543 phalloidin have been delivered successively into fibroblast cells, which enables us to monitor the distinct roles of vinculin and F-actin in cell adhesion and migration as well as their possible interplay during these processes. Compared to conventional bulk electroporation and staining methods, the new system offers advantages such as low-voltage operation, cellular level manipulation and testing, fast and adjustable transfection/staining and real-time monitoring; the new system therefore could be useful in different biophysical studies in the future. View Full-Text
Keywords: electroporation; membrane resealing; electrotransfection; live cell staining; cell adhesion electroporation; membrane resealing; electrotransfection; live cell staining; cell adhesion
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MDPI and ACS Style

Zhang, Y.; Yan, Z.; Xia, X.; Lin, Y. A Novel Electroporation System for Living Cell Staining and Membrane Dynamics Interrogation. Micromachines 2020, 11, 767.

AMA Style

Zhang Y, Yan Z, Xia X, Lin Y. A Novel Electroporation System for Living Cell Staining and Membrane Dynamics Interrogation. Micromachines. 2020; 11(8):767.

Chicago/Turabian Style

Zhang, Yuanjun; Yan, Zishen; Xia, Xingyu; Lin, Yuan. 2020. "A Novel Electroporation System for Living Cell Staining and Membrane Dynamics Interrogation" Micromachines 11, no. 8: 767.

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