Next Article in Journal
Cell Culture on Low-Fluorescence and High-Resolution Photoresist
Previous Article in Journal
Bistable Threshold Humidity Sensor Switch with Rectangular Bimorph Bending Plate
Open AccessArticle

Analysis of the Binding of Analyte-Receptor in a Micro-Fluidic Channel for a Biosensor Based on Brownian Motion

1
School of Mechanical and Aerospace Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Korea
2
Department of Nano Manufacturing Technology, Korea Institute of Machinery and Materials, 156 Gajeongbuk-Ro, Yusung-Gu, Daejeon 34103, Korea
*
Author to whom correspondence should be addressed.
Micromachines 2020, 11(6), 570; https://doi.org/10.3390/mi11060570
Received: 6 May 2020 / Revised: 29 May 2020 / Accepted: 2 June 2020 / Published: 3 June 2020
This study experimentally analyses the binding characteristics of analytes mixed in liquid samples flowing along a micro-channel to the receptor fixed on the wall of the micro-channel to provide design tools and data for a microfluidic-based biosensor. The binding or detection characteristics are analyzed experimentally by counting the number of analytes bound to the receptor, with sample analyte concentration, sample flow rate, and the position of the receptor along the micro-channel length as the main variables. A mathematical model is also proposed to predict the number of analytes transported and bound to the receptor based on a probability density function for Brownian motion. The coefficient in the mathematical model is obtained by using a dimensionless mathematical model and the experimental results. The coefficient remains valid for all different conditions of the sample analyte concentration, flow rate, and the position of the receptor, which implies the possibility of deriving a generalized model. Based on the mathematical model derived from mathematical and experimental analysis on the detection characteristics of the microfluidic-based biosensor depending on previously mentioned variables and the height of the micro-channel, this study suggests a design for a microfluidic-based biosensor by predicting the binding efficiency according to the channel height. The results show the binding efficiency increases as the flow rate decreases and as the receptor is placed closer to the sample-injecting inlet, but is unaffected by sample concentration. View Full-Text
Keywords: biosensor; specific binding of analyte-receptor; transport model for micro-fluidic channel based on Brownian motion biosensor; specific binding of analyte-receptor; transport model for micro-fluidic channel based on Brownian motion
Show Figures

Figure 1

MDPI and ACS Style

Choi, S.; Lee, W.I.; Lee, G.H.; Yoo, Y.-E. Analysis of the Binding of Analyte-Receptor in a Micro-Fluidic Channel for a Biosensor Based on Brownian Motion. Micromachines 2020, 11, 570.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop