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Open AccessArticle

A Simple and Rapid Fungal DNA Isolation Assay Based on ZnO Nanoparticles for the Diagnosis of Invasive Aspergillosis

1
Department of Convergence Medicine, Asan Medical Institute of Convergence Science and Technology (AMIST), University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul 05505, Korea
2
Department of Bionanotechnology, Gachon University, Gyeonggi-do 13120, Korea
3
Department of Infectious Disease, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul 05505, Korea
*
Authors to whom correspondence should be addressed.
These authors (Z.Q. & H.L.) contributed equally to this work.
Micromachines 2020, 11(5), 515; https://doi.org/10.3390/mi11050515
Received: 21 April 2020 / Revised: 13 May 2020 / Accepted: 18 May 2020 / Published: 19 May 2020
(This article belongs to the Special Issue Molecular Diagnostic Devices and Clinical Applications)
Invasive aspergillosis (IA) is an important cause of morbidity and mortality among immunocompromised people. Imaging and specimen tests used in the clinical diagnosis of aspergillosis with weak and indistinct defects leads to delay in the treatment of early aspergillosis patients. The developing molecular techniques provide a new method for the aspergillosis diagnosis. However, the existing methods are complex, time-consuming and may even be potentially hazardous. In this study, we developed a simple and rapid Aspergillus fumigatus spores DNA isolation assay using synthesized zinc oxide (ZnO). ZnO nanoparticles were used to take the place of the traditional commercial lysis buffer. The quality and quantity of the extracted DNA were sufficient for further diagnostics with polymerase chain reaction (PCR) analysis. This method offers easy, green, and economic alternative DNA isolation for the diagnosis of invasive aspergillosis.
Keywords: molecular diagnostics; medical device; clinical applications; invasive aspergillosis; zinc oxide nanoparticle molecular diagnostics; medical device; clinical applications; invasive aspergillosis; zinc oxide nanoparticle
MDPI and ACS Style

Qiao, Z.; Liu, H.; Noh, G.S.; Koo, B.; Zou, Q.; Yun, K.; Jang, Y.O.; Kim, S.-H.; Shin, Y. A Simple and Rapid Fungal DNA Isolation Assay Based on ZnO Nanoparticles for the Diagnosis of Invasive Aspergillosis. Micromachines 2020, 11, 515.

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