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Open AccessArticle

Label-Free, High-Throughput Assay of Human Dendritic Cells from Whole-Blood Samples with Microfluidic Inertial Separation Suitable for Resource-Limited Manufacturing

1
Department of Electrical & Electronics Engineering, Universiti Teknologi PETRONAS, Perak 32610, Malaysia
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Department of Electronics and Communication Engineering, C. Abdul Hakeem College of Engineering and Technology, Melvisharam, Tamil Nadu 632509, India
3
Head and Neck Cancer Research Group, Cancer Research Malaysia, Selangor 47500, Malaysia
4
Department of Oral & Maxillofacial Clinical Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur 50603, Malaysia
*
Author to whom correspondence should be addressed.
Micromachines 2020, 11(5), 514; https://doi.org/10.3390/mi11050514
Received: 27 February 2020 / Revised: 31 March 2020 / Accepted: 5 April 2020 / Published: 19 May 2020
(This article belongs to the Special Issue Microfluidics Technologies for Cell-based Assays)
Microfluidics technology has not impacted the delivery and accessibility of point-of-care health services, like diagnosing infectious disease, monitoring health or delivering interventions. Most microfluidics prototypes in academic research are not easy to scale-up with industrial-scale fabrication techniques and cannot be operated without complex manipulations of supporting equipment and additives, such as labels or reagents. We propose a label- and reagent-free inertial spiral microfluidic device to separate red blood, white blood and dendritic cells from blood fluid, for applications in health monitoring and immunotherapy. We demonstrate that using larger channel widths, in the range of 200 to 600 µm, allows separation of cells into multiple focused streams, according to different size ranges, and we utilize a novel technique to collect the closely separated focused cell streams, without constricting the channel. Our contribution is a method to adapt spiral inertial microfluidic designs to separate more than two cell types in the same device, which is robust against clogging, simple to operate and suitable for fabrication and deployment in resource-limited populations. When tested on actual human blood cells, 77% of dendritic cells were separated and 80% of cells remained viable after our assay. View Full-Text
Keywords: inertial spiral microfluidics; dendritic cell separation; resource-limited microfluidics inertial spiral microfluidics; dendritic cell separation; resource-limited microfluidics
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Caffiyar, M.Y.; Lim, K.P.; Basha, I.H.K.; Hamid, N.H.; Cheong, S.C.; Ho, E.T.W. Label-Free, High-Throughput Assay of Human Dendritic Cells from Whole-Blood Samples with Microfluidic Inertial Separation Suitable for Resource-Limited Manufacturing. Micromachines 2020, 11, 514.

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