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Article

Integrated On-Chip 3D Vascular Network Culture under Hypoxia

1
Departamento de Química Analítica, Facultad de Química, Universidad Nacional Autónoma de México, Av. Universidad 3000, Ciudad de Mexico 04510, Mexico
2
Department of Mechanical Engineering, College of Engineering, Shibaura Institute of Technology, 3-7-5 Toyosu, Koto-ku, Tokyo 135-8548, Japan
3
Digital Manufacturing and Design Centre, Singapore University of Technology and Design, 8 Somapah Road, Singapore 487372, Singapore
*
Author to whom correspondence should be addressed.
Micromachines 2020, 11(5), 475; https://doi.org/10.3390/mi11050475
Received: 21 March 2020 / Revised: 28 April 2020 / Accepted: 28 April 2020 / Published: 30 April 2020
(This article belongs to the Special Issue Microfluidic Platforms for Cell Culture and Investigations)
We developed a portable device made of poly(dimethylsiloxane) (PDMS)/polymethylmethacrylate (PMMA) for long-term 3D cell culture of vascular endothelial cells for the development of a vascular network and evaluated the device under different transitions between normoxia and hypoxia with good optical accessibility. The combination of a nested reservoir device and a bicarbonate/ascorbate buffer system accomplished on-chip incubation with 4.91 ± 0.86% pO2 and 5.19 ± 1.70% pCO2 for up to 10 days. Seventy-two hours of normoxic incubation preceding hypoxic culture increased the cell viability, network formation, and size and stability of the resulting lumens compared with those completely maintained in normoxia for the same total duration. We employed different parameters of the network (e.g., total mesh area, total length, number of branches, among others) for the comparison of different oxygen treatments in the device. The differential effect of hypoxic conditions based on the maturity of the vessels may be used as an external factor to improve vascular development in vitro. View Full-Text
Keywords: 3D cell culture; hypoxia; angiogenesis; portable cell culture device; vascular network; normoxic/hypoxic transition 3D cell culture; hypoxia; angiogenesis; portable cell culture device; vascular network; normoxic/hypoxic transition
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MDPI and ACS Style

Olmedo-Suárez, M.Á.; Sekiguchi, T.; Takano, A.; Cañizares-Macías, M.d.P.; Futai, N. Integrated On-Chip 3D Vascular Network Culture under Hypoxia. Micromachines 2020, 11, 475. https://doi.org/10.3390/mi11050475

AMA Style

Olmedo-Suárez MÁ, Sekiguchi T, Takano A, Cañizares-Macías MdP, Futai N. Integrated On-Chip 3D Vascular Network Culture under Hypoxia. Micromachines. 2020; 11(5):475. https://doi.org/10.3390/mi11050475

Chicago/Turabian Style

Olmedo-Suárez, Miguel Á., Tomohiro Sekiguchi, Atsushi Takano, Maria d.P. Cañizares-Macías, and Nobuyuki Futai. 2020. "Integrated On-Chip 3D Vascular Network Culture under Hypoxia" Micromachines 11, no. 5: 475. https://doi.org/10.3390/mi11050475

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