Size Sorting of Exosomes by Tuning the Thicknesses of the Electric Double Layers on a Micro-Nanofluidic Device

Fujiwara, Satoko; Morikawa, Kyojiro; Endo, Tatsuro; Hisamoto, Hideaki; Sueyoshi, Kenji

doi:10.3390/mi11050458

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Size Sorting of Exosomes by Tuning the Thicknesses of the Electric Double Layers on a Micro-Nanofluidic Device

by

Satoko Fujiwara

¹,

Kyojiro Morikawa

²

,

Tatsuro Endo

¹

,

Hideaki Hisamoto

¹ and

Kenji Sueyoshi

^1,3,*

¹

Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, Osaka 599-8531, Japan

²

Department of Applied Chemistry, School of Engineering, University of Tokyo, Tokyo 113-0033, Japan

³

Japan Science and Technology Agency (JST), Precursory Research for Embryonic Science and Technology (PRESTO), Tokyo 102-8666, Japan

^*

Author to whom correspondence should be addressed.

Micromachines 2020, 11(5), 458; https://doi.org/10.3390/mi11050458

Submission received: 15 April 2020 / Revised: 26 April 2020 / Accepted: 27 April 2020 / Published: 28 April 2020

(This article belongs to the Special Issue Advances in Nanofluidics)

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Abstract

Exosomes, a type of extracellular vesicle with a diameter of 30–150 nm, perform key biological functions such as intercellular communication. Recently, size sorting of exosomes has received increasing attention in order to clarify the correlation between their size and components. However, such sorting remains extremely difficult. Here, we propose to sort their size by controlling their electrokinetic migration in nanochannels in a micro-nanofluidic device, which is achieved by tuning the thickness of the electric double layers in the nanochannels. This approach was demonstrated experimentally for exosomes smaller than 250 nm. Using different running buffer concentrations (1 × 10⁻³, 1 × 10⁻⁴, and 1 × 10⁻⁵ M), most of the exosomes larger than 140, 110, and 80 nm were successfully cut off at the downstream of the nanochannels, respectively. Therefore, it is clarified that the proposed method is applicable for the size sorting of exosomes.

Keywords: exosomes; size sorting; electric double layers; nanochannels; micro-nanofluidic device

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MDPI and ACS Style

Fujiwara, S.; Morikawa, K.; Endo, T.; Hisamoto, H.; Sueyoshi, K. Size Sorting of Exosomes by Tuning the Thicknesses of the Electric Double Layers on a Micro-Nanofluidic Device. Micromachines 2020, 11, 458. https://doi.org/10.3390/mi11050458

AMA Style

Fujiwara S, Morikawa K, Endo T, Hisamoto H, Sueyoshi K. Size Sorting of Exosomes by Tuning the Thicknesses of the Electric Double Layers on a Micro-Nanofluidic Device. Micromachines. 2020; 11(5):458. https://doi.org/10.3390/mi11050458

Chicago/Turabian Style

Fujiwara, Satoko, Kyojiro Morikawa, Tatsuro Endo, Hideaki Hisamoto, and Kenji Sueyoshi. 2020. "Size Sorting of Exosomes by Tuning the Thicknesses of the Electric Double Layers on a Micro-Nanofluidic Device" Micromachines 11, no. 5: 458. https://doi.org/10.3390/mi11050458

APA Style

Fujiwara, S., Morikawa, K., Endo, T., Hisamoto, H., & Sueyoshi, K. (2020). Size Sorting of Exosomes by Tuning the Thicknesses of the Electric Double Layers on a Micro-Nanofluidic Device. Micromachines, 11(5), 458. https://doi.org/10.3390/mi11050458

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Size Sorting of Exosomes by Tuning the Thicknesses of the Electric Double Layers on a Micro-Nanofluidic Device

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