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Open AccessArticle

Microfluidic Device for Microinjection of Caenorhabditis elegans

1
Department of Mechanical Engineering, McMaster University, Hamilton, ON L8S 4L8, Canada
2
Department of Biology, McMaster University, Hamilton, ON L8S 4L8, Canada
*
Author to whom correspondence should be addressed.
Micromachines 2020, 11(3), 295; https://doi.org/10.3390/mi11030295
Received: 9 February 2020 / Revised: 6 March 2020 / Accepted: 9 March 2020 / Published: 11 March 2020
(This article belongs to the Special Issue 10th Anniversary of Micromachines)
Microinjection is an established and reliable method to deliver transgenic constructs and other reagents to specific locations in C. elegans worms. Specifically, microinjection of a desired DNA construct into the distal gonad is the most widely used method to generate germ-line transformation of C. elegans. Although, current C. elegans microinjection method is effective to produce transgenic worms, it requires expensive multi degree of freedom (DOF) micromanipulator, careful injection alignment procedure and skilled operator, all of which make it slow and not suitable for scaling to high throughput. A few microfabricated microinjectors have been developed recently to address these issues. However, none of them are capable of immobilizing a freely mobile animal such as C. elegans worm using a passive immobilization mechanism. Here, a microfluidic microinjector was developed to passively immobilize a freely mobile animal such as C. elegans and simultaneously perform microinjection by using a simple and fast mechanism for needle actuation. The entire process of the microinjection takes ~30 s which includes 10 s for worm loading and aligning, 5 s needle penetration, 5 s reagent injection and 5 s worm unloading. The device is suitable for high-throughput and can be potentially used for creating transgenic C. elegans. View Full-Text
Keywords: C. elegans; microinjection; microfluidics; compliant mechanism C. elegans; microinjection; microfluidics; compliant mechanism
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MDPI and ACS Style

Ghaemi, R.; Tong, J.; Gupta, B.P.; Selvaganapathy, P.R. Microfluidic Device for Microinjection of Caenorhabditis elegans. Micromachines 2020, 11, 295.

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