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Open AccessArticle

A Novel Microfluidic Device Integrated with Chitosan-Modified Capillaries for Rapid ZIKV Detection

1
Optoelectronic Application Technology Research Center, Institute of Applied Technology, Hefei Institutes of Physical Science, Chinese Academy of Science, Hefei 230031, China
2
University of Science and Technology of China, Hefei 230026, China
3
Institute of Optical Engineering, Anhui Normal University, No. 1, Beijing East Road, Wuhu 241000, China
*
Authors to whom correspondence should be addressed.
Micromachines 2020, 11(2), 186; https://doi.org/10.3390/mi11020186
Received: 30 December 2019 / Revised: 29 January 2020 / Accepted: 3 February 2020 / Published: 11 February 2020
(This article belongs to the Special Issue Point-of-Care Devices)
The outbreak of Zika virus (ZIKV) has posed a great challenge to public health in recent years. To address the urgent need of ZIKV RNA assays, we integrate the microfluidic chip embedded with chitosan-modified silicon dioxide capillaries, smartphone-based detection unit to be a C3-system for the rapid extraction and detection of ZIKV RNA. The C3-system is characterized by: (1) four chitosan-modified silicon dioxide capillaries integrated in the microfluidic chip for target ZIKV RNA enrichment and “in situ PCR” (polymerase chain reaction) amplification; (2) smartphone-based point of care (POC) device consisting of a pneumatic subsystem for controlling the nucleic acid extraction processes in the microfluidic chip, a heating subsystem for sample lysis and PCR amplification, and an optical subsystem for signal acquisition. The entire detection processes including sample lysis, ZIKV RNA enrichment, and reverse-transcription polymerase chain reaction (RT-PCR) is achieved in the microfluidic chip. Moreover, PCR buffers can be directly loaded into the chitosan-modified silicon dioxide capillaries for “in situ PCR”, in which the captured ZIKV RNA is directly used for downstream PCR without any loss. ZIKV RNA extracted by the C3-system can be successfully recovered at very low concentrations of 50 transducing units (TU)/mL from crude human saliva. This means that our method of detecting viremia in patients infected with ZIKV is reliable.
Keywords: in situ PCR; molecular diagnosis; microfluidic chip; POC device; ZIKV in situ PCR; molecular diagnosis; microfluidic chip; POC device; ZIKV
MDPI and ACS Style

Zhu, X.; Zhao, J.; Hu, A.; Pan, J.; Deng, G.; Hua, C.; Zhu, C.; Liu, Y.; Yang, K.; Zhu, L. A Novel Microfluidic Device Integrated with Chitosan-Modified Capillaries for Rapid ZIKV Detection. Micromachines 2020, 11, 186.

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