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Open AccessArticle

Continuous Microfluidic Purification of DNA Using Magnetophoresis

1
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
2
Shenzhen Research Institute of Southeast University, Shenzhen Virtual University Park, Shenzhen 518000, China
*
Author to whom correspondence should be addressed.
Micromachines 2020, 11(2), 187; https://doi.org/10.3390/mi11020187
Received: 7 January 2020 / Revised: 5 February 2020 / Accepted: 7 February 2020 / Published: 11 February 2020
(This article belongs to the Special Issue Electrokinetics in Micro-/nanofluidic Devices)
Automatic microfluidic purification of nucleic acid is predictable to reduce the input of original samples and improve the throughput of library preparation for sequencing. Here, we propose a novel microfluidic system using an external NdFeB magnet to isolate DNA from the polymerase chain reaction (PCR) mixture. The DNA was purified and isolated when the DNA-carrying beads transported to the interface of multi-laminar flow under the influence of magnetic field. Prior to the DNA recovery experiments, COMSOL simulations were carried out to study the relationship between trajectory of beads and magnet positions as well as fluid velocities. Afterwards, the experiments to study the influence of varying velocities and input of samples on the DNA recovery were conducted. Compared to experimental results, the relative error of the final position of beads is less than 10%. The recovery efficiency decreases with increase of input or fluid velocity, and the maximum DNA recovery efficiency is 98.4% with input of l00 ng DNA at fluid velocity of 1.373 mm/s. The results show that simulations significantly reduce the time for parameter adjustment in experiments. In addition, this platform uses a basic two-layer chip to realize automatic DNA isolation without any other liquid switch value or magnet controller.
Keywords: microfluidics; DNA purification and extraction; magnetophoresis; multi-laminar flow; COMSOL simulation microfluidics; DNA purification and extraction; magnetophoresis; multi-laminar flow; COMSOL simulation
MDPI and ACS Style

Xu, Y.; Zhang, Z.; Su, Z.; Zhou, X.; Han, X.; Liu, Q. Continuous Microfluidic Purification of DNA Using Magnetophoresis. Micromachines 2020, 11, 187.

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