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Open AccessArticle

Nanosensors-Assisted Quantitative Analysis of Biochemical Processes in Droplets

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Max Bergmann Center of Biomaterials and Institute for Materials Science, Technische Universität Dresden, 01069 Dresden, Germany
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Technische Universität Dresden, Center for Advancing Electronics Dresden, 01062 Dresden, Germany
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Department of Creative IT Engineering, Pohang University of Science and Technology, 37673 Pohang, Korea
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Dresden Center for Computational Materials Science, 01062 Dresden, Germany
*
Authors to whom correspondence should be addressed.
Micromachines 2020, 11(2), 138; https://doi.org/10.3390/mi11020138
Received: 30 December 2019 / Revised: 20 January 2020 / Accepted: 22 January 2020 / Published: 26 January 2020
(This article belongs to the Special Issue Nanomaterials-Based Biosensors)
Here, we present a miniaturized lab-on-a-chip detecting system for an all-electric and label-free analysis of the emulsion droplets incorporating the nanoscopic silicon nanowires-based field-effect transistors (FETs). We specifically focus on the analysis of β-galactosidase e.g., activity, which is an important enzyme of the glycolysis metabolic pathway. Furthermore, the efficiency of the synthesis and action of β-galactosidase can be one of the markers for several diseases, e.g., cancer, hyper/hypoglycemia, cell senescence, or other disruptions in cell functioning. We measure the reaction and reaction kinetics-associated shift of the source-to-drain current Isd in the system, which is caused by the change of the ionic strength of the microenvironment. With these results, we demonstrate that the ion-sensitive FETs are able to sense the interior of the aqueous reactors; thus, the conjunction of miniature nanosensors and droplet-based microfluidic systems conceptually opens a new route toward a sensitive, optics-less analysis of biochemical processes. View Full-Text
Keywords: silicon nanowire-based field-effect transistor; nanosensor; droplet-based microfluidics; point-of-care diagnostics; enzymatic reaction; β-galactosidase assay; lab-on-a-chip silicon nanowire-based field-effect transistor; nanosensor; droplet-based microfluidics; point-of-care diagnostics; enzymatic reaction; β-galactosidase assay; lab-on-a-chip
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MDPI and ACS Style

Belyaev, D.; Schütt, J.; Ibarlucea, B.; Rim, T.; Baraban, L.; Cuniberti, G. Nanosensors-Assisted Quantitative Analysis of Biochemical Processes in Droplets. Micromachines 2020, 11, 138.

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