Next Article in Journal
Editorial for the Special Issue on Implantable Microdevices
Next Article in Special Issue
A Misalignment Optical Guiding Module for Power Generation Enhancement of Fixed-Type Photovoltaic Systems
Previous Article in Journal
Sustainable Fully Printed UV Sensors on Cork Using Zinc Oxide/Ethylcellulose Inks
Previous Article in Special Issue
A Gas Mixture Prediction Model Based on the Dynamic Response of a Metal-Oxide Sensor
Open AccessArticle

Microfluidic-Based Mechanical Phenotyping of Androgen-Sensitive and Non-sensitive Prostate Cancer Cells Lines

1
School of Mechatronics Engineering and Automation, Shanghai University, Shanghai 200444, China
2
Fudan Institute of Urology, Fudan University, Shanghai 200433, China
*
Authors to whom correspondence should be addressed.
Micromachines 2019, 10(9), 602; https://doi.org/10.3390/mi10090602
Received: 14 July 2019 / Revised: 7 September 2019 / Accepted: 10 September 2019 / Published: 12 September 2019
(This article belongs to the Special Issue Advanced MEMS/NEMS Technology, Volume II)
Cell mechanical properties have been identified to characterize cells pathologic states. Here, we report our work on high-throughput mechanical phenotyping of androgen-sensitive and non-sensitive human prostate cancer cell lines based on a morphological rheological microfluidic method. The theory for extracting cells’ elastic modulus from their deformation and area, and the used experimental parameters were analyzed. The mechanical properties of three types of prostate cancer cells lines with different sensitivity to androgen including LNCaP, DU145, and PC3 were quantified. The result shows that LNCaP cell was the softest, DU145 was the second softest, and PC3 was the stiffest. Furthermore, atomic force microscopy (AFM) was used to verify the effectiveness of this high-throughput morphological rheological method. View Full-Text
Keywords: cellular mechanics; cell deformability; single-cell analysis; high-throughput; microfluidic; morphological rheology cellular mechanics; cell deformability; single-cell analysis; high-throughput; microfluidic; morphological rheology
Show Figures

Figure 1

MDPI and ACS Style

Liu, N.; Du, P.; Xiao, X.; Liu, Y.; Peng, Y.; Yang, C.; Yue, T. Microfluidic-Based Mechanical Phenotyping of Androgen-Sensitive and Non-sensitive Prostate Cancer Cells Lines. Micromachines 2019, 10, 602.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop