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Open AccessArticle

A Passive Microfluidic Device for Chemotaxis Studies

1
Department of Experimental and Clinical Medicine, University “Magna Graecia” of Catanzaro, 88100 Catanzaro, Italy
2
Department of Chemical and Biochemical Engineering, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark
3
Physical Science and Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia
*
Author to whom correspondence should be addressed.
Micromachines 2019, 10(8), 551; https://doi.org/10.3390/mi10080551
Received: 10 July 2019 / Revised: 11 August 2019 / Accepted: 16 August 2019 / Published: 20 August 2019
This work presents a disposable passive microfluidic system, allowing chemotaxis studies, through the generation of a concentration gradient. The device can handle liquid flows without an external supply of pressure or electric gradients, but simply using gravity force. It is able to ensure flow rates of 10 µL/h decreasing linearly with 2.5% in 24 h. The device is made of poly(methylmethacrylate) (PMMA), a biocompatible material, and it is fabricated by micro-milling and solvent assisted bonding. It is assembled into a mini incubator, designed properly for cell biology studies in passive microfluidic devices, which provides control of temperature and humidity levels, a contamination-free environment for cells with air and 5% of CO2. Furthermore, the mini incubator can be mounted on standard inverted optical microscopes. By using our microfluidic device integrated into the mini incubator, we are able to evaluate and follow in real-time the migration of any cell line to a chemotactic agent. The device is validated by showing cell migration at a rate of 0.36 µm/min, comparable with the rates present in scientific literature. View Full-Text
Keywords: chemotaxis; passive microfluidic device; diffusion; mini incubator chemotaxis; passive microfluidic device; diffusion; mini incubator
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MDPI and ACS Style

Coluccio, M.L.; D’Attimo, M.A.; Cristiani, C.M.; Candeloro, P.; Parrotta, E.; Dattola, E.; Guzzi, F.; Cuda, G.; Lamanna, E.; Carbone, E.; Krühne, U.; Di Fabrizio, E.; Perozziello, G. A Passive Microfluidic Device for Chemotaxis Studies. Micromachines 2019, 10, 551. https://doi.org/10.3390/mi10080551

AMA Style

Coluccio ML, D’Attimo MA, Cristiani CM, Candeloro P, Parrotta E, Dattola E, Guzzi F, Cuda G, Lamanna E, Carbone E, Krühne U, Di Fabrizio E, Perozziello G. A Passive Microfluidic Device for Chemotaxis Studies. Micromachines. 2019; 10(8):551. https://doi.org/10.3390/mi10080551

Chicago/Turabian Style

Coluccio, Maria L.; D’Attimo, Maria A.; Cristiani, Costanza M.; Candeloro, Patrizio; Parrotta, Elvira; Dattola, Elisabetta; Guzzi, Francesco; Cuda, Giovanni; Lamanna, Ernesto; Carbone, Ennio; Krühne, Ulrich; Di Fabrizio, Enzo; Perozziello, Gerardo. 2019. "A Passive Microfluidic Device for Chemotaxis Studies" Micromachines 10, no. 8: 551. https://doi.org/10.3390/mi10080551

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