Next Article in Journal / Special Issue
A Thermal Tactile Sensation Display with Controllable Thermal Conductivity
Previous Article in Journal
Porous Alginate Scaffolds Assembled Using Vaterite CaCO3 Crystals
Previous Article in Special Issue
Magneto-Impedance Sensor Driven by 400 MHz Logarithmic Amplifier
Open AccessFeature PaperArticle

Temporal Observation of Adipocyte Microfiber Using Anchoring Device

1
Center for International Research on Integrative Biomedical Systems (CIBiS), Institute of Industrial Science (IIS), The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan
2
Department of Mechano-Informatics, Graduate School of Information Science and Technology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
3
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
4
International Research Center for Neurointelligence (WPI-IRCN), The University of Tokyo Institutes for Advanced Study (UTIAS), The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
*
Author to whom correspondence should be addressed.
Micromachines 2019, 10(6), 358; https://doi.org/10.3390/mi10060358
Received: 11 May 2019 / Revised: 27 May 2019 / Accepted: 28 May 2019 / Published: 29 May 2019
In this paper, we propose an anchoring device with pillars to immobilize an adipocyte microfiber that has a fiber-shaped adipocyte tissue covered by an alginate gel shell. Because the device enabled the immobilization of the microfiber in a culture dish even after its transportation and the exchange of the culture medium, we can easily track the specific positions of the microfiber for a long period. Owing to the characteristics of the anchoring device, we successfully performed temporal observations of the microfiber on the device for a month to investigate the function and morphology of three-dimensional cultured adipocytes. Furthermore, to demonstrate the applicability of the anchoring device to drug testing, we evaluated the lipolysis of the microfiber’s adipocytes by applying reagents with an anti-obesity effect. Therefore, we believe that the anchoring device with the microfiber will be a useful tool for temporal biochemical analyses. View Full-Text
Keywords: microfluidics; biofabrication; adipose tissue; lipolysis microfluidics; biofabrication; adipose tissue; lipolysis
Show Figures

Figure 1

MDPI and ACS Style

Yokomizo, A.; Morimoto, Y.; Nishimura, K.; Takeuchi, S. Temporal Observation of Adipocyte Microfiber Using Anchoring Device. Micromachines 2019, 10, 358.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop