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A Novel Biodegradable Multilayered Bioengineered Vascular Construct with a Curved Structure and Multi-Branches

1
School of Mechatronic Engineering and Automation, Shanghai University, Shanghai 200444, China
2
School of Mechanical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
*
Authors to whom correspondence should be addressed.
Micromachines 2019, 10(4), 275; https://doi.org/10.3390/mi10040275
Received: 15 March 2019 / Revised: 17 April 2019 / Accepted: 21 April 2019 / Published: 24 April 2019
(This article belongs to the Special Issue 3D Printing for Tissue Engineering and Regenerative Medicine)
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Abstract

Constructing tissue engineered vascular grafts (TEVG) is of great significance for cardiovascular research. However, most of the fabrication techniques are unable to construct TEVG with a bifurcated and curved structure. This paper presents multilayered biodegradable TEVGs with a curved structure and multi-branches. The technique combined 3D printed molds and casting hydrogel and sacrificial material to create vessel-mimicking constructs with customizable structural parameters. Compared with other fabrication methods, the proposed technique can create more native-like 3D geometries. The diameter and wall thickness of the fabricated constructs can be independently controlled, providing a feasible approach for TEVG construction. Enzymatically-crosslinked gelatin was used as the material of the constructs. The mechanical properties and thermostability of the constructs were evaluated. Fluid-structure interaction simulations were conducted to examine the displacement of the construct’s wall when blood flows through it. Human umbilical vein endothelial cells (HUVECs) were seeded on the inner channel of the constructs and cultured for 72 h. The cell morphology was assessed. The results showed that the proposed technique had good application potentials, and will hopefully provide a novel technological approach for constructing integrated vasculature for tissue engineering. View Full-Text
Keywords: bioengineered vascular constructs; curved structure; multi-branches; enzymatically-crosslinked; tissue engineering bioengineered vascular constructs; curved structure; multi-branches; enzymatically-crosslinked; tissue engineering
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Liu, Y.; Zhang, Y.; Jiang, W.; Peng, Y.; Luo, J.; Xie, S.; Zhong, S.; Pu, H.; Liu, N.; Yue, T. A Novel Biodegradable Multilayered Bioengineered Vascular Construct with a Curved Structure and Multi-Branches. Micromachines 2019, 10, 275.

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