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Micromachines 2019, 10(2), 116; https://doi.org/10.3390/mi10020116

Microfluidic High-Migratory Cell Collector Suppressing Artifacts Caused by Microstructures

1
Department of Mechanical Engineering, School of Engineering, Tokyo Institute of Technology, Kanagawa 226-8503, Japan
2
Department of Mechano-Micro Engineering, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, Kanagawa 226-8503, Japan
3
Department of Life Science and Technology, School of Life Science and Technology, Tokyo Institute of Technology, Kanagawa 226-8503, Japan
*
Author to whom correspondence should be addressed.
Received: 17 January 2019 / Revised: 1 February 2019 / Accepted: 7 February 2019 / Published: 11 February 2019
(This article belongs to the Section B:Biology)
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Abstract

The small number of high-migratory cancer cells in a cell population make studies on high-migratory cancer cells difficult. For the development of migration assays for such cancer cells, several microfluidic devices have been developed. However, they measure migration that is influenced by microstructures and they collect not only high-migratory cells, but also surrounding cells. In order to find high-migratory cells in cell populations while suppressing artifacts and to collect these cells while minimizing damages, we developed a microfluidic high-migratory cell collector with the ability to sort cancer cells according to cellular migration and mechanical detachment. High-migratory cancer cells travel further from the starting line when all of the cells are seeded on the same starting line. The high-migratory cells are detached using a stretch of cell adhesive surface using a water-driven balloon actuator. Using this cell collector, we selected high-migratory HeLa cells that migrated about 100 μm in 12 h and collected the cells. View Full-Text
Keywords: high-migratory cell; microfluidic cell collector; balloon; migration assay high-migratory cell; microfluidic cell collector; balloon; migration assay
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Ishida, T.; Shimamoto, T.; Kaminaga, M.; Kuchimaru, T.; Kizaka-Kondoh, S.; Omata, T. Microfluidic High-Migratory Cell Collector Suppressing Artifacts Caused by Microstructures. Micromachines 2019, 10, 116.

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