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Open AccessArticle

Tetrafluoroethylene-Propylene Elastomer for Fabrication of Microfluidic Organs-on-Chips Resistant to Drug Absorption

1
Department of Micro Engineering, Kyoto University, Kyoto 615-8540, Japan
2
AGC Inc, Tokyo 100-8405, Japan
3
Hakubi Center for Advanced Research, Kyoto University, Kyoto 615-8540, Japan
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Micromachines 2019, 10(11), 793; https://doi.org/10.3390/mi10110793
Received: 26 August 2019 / Revised: 8 November 2019 / Accepted: 15 November 2019 / Published: 19 November 2019
(This article belongs to the Special Issue Organs-on-chips)
Organs-on-chips are microfluidic devices typically fabricated from polydimethylsiloxane (PDMS). Since PDMS has many attractive properties including high optical clarity and compliance, PDMS is very useful for cell culture applications; however, PDMS possesses a significant drawback in that small hydrophobic molecules are strongly absorbed. This drawback hinders widespread use of PDMS-based devices for drug discovery and development. Here, we describe a microfluidic cell culture system made of a tetrafluoroethylene-propylene (FEPM) elastomer. We demonstrated that FEPM does not absorb small hydrophobic compounds including rhodamine B and three types of drugs, nifedipine, coumarin, and Bay K8644, whereas PDMS absorbs them strongly. The device consists of two FEPM layers of microchannels separated by a thin collagen vitrigel membrane. Since FEPM is flexible and biocompatible, this microfluidic device can be used to culture cells while applying mechanical strain. When human umbilical vein endothelial cells (HUVECs) were subjected to cyclic strain (~10%) for 4 h in this device, HUVECs reoriented and aligned perpendicularly in response to the cyclic stretch. Moreover, we demonstrated that this device can be used to replicate the epithelial–endothelial interface as well as to provide physiological mechanical strain and fluid flow. This method offers a robust platform to produce organs-on-chips for drug discovery and development. View Full-Text
Keywords: organs-on-chips; microfluidics; drug absorption; fluoroelastomer organs-on-chips; microfluidics; drug absorption; fluoroelastomer
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Sano, E.; Mori, C.; Matsuoka, N.; Ozaki, Y.; Yagi, K.; Wada, A.; Tashima, K.; Yamasaki, S.; Tanabe, K.; Yano, K.; Torisawa, Y.-S. Tetrafluoroethylene-Propylene Elastomer for Fabrication of Microfluidic Organs-on-Chips Resistant to Drug Absorption. Micromachines 2019, 10, 793.

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