Next Article in Journal
Bee Venom Protects against Rotenone-Induced Cell Death in NSC34 Motor Neuron Cells
Next Article in Special Issue
Studies on the Bioavailability of Deoxynivalenol (DON) and DON Sulfonate (DONS) 1, 2, and 3 in Pigs Fed with Sodium Sulfite-Treated DON-Contaminated Maize
Previous Article in Journal
Staphylococcus epidermidis and Staphylococcus haemolyticus: Molecular Detection of Cytotoxin and Enterotoxin Genes
Previous Article in Special Issue
Zearalenone in the Intestinal Tissues of Immature Gilts Exposed per os to Mycotoxins
Open AccessArticle

Detection of N-(1-deoxy-d-fructos-1-yl) Fumonisins B2 and B3 in Corn by High-Resolution LC-Orbitrap MS

1
National Agriculture and Food Research Organization (NARO), National Food Research Institute, 2-1-12 Kannon-dai, Tsukuba-shi, Ibaraki 305-8642, Japan
2
Thermo Fisher Scientific, C-2F, 3-9 Moriya-cho, Yokohama-shi, Kanagawa 221-0022, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Sven Dänicke
Toxins 2015, 7(9), 3700-3714; https://doi.org/10.3390/toxins7093700
Received: 30 June 2015 / Revised: 3 September 2015 / Accepted: 7 September 2015 / Published: 16 September 2015
(This article belongs to the Collection Fusarium Toxins – Relevance for Human and Animal Health)
The existence of glucose conjugates of fumonisin B2 (FB2) and fumonisin B3 (FB3) in corn powder was confirmed for the first time. These “bound-fumonisins” (FB2 and FB3 bound to glucose) were identified as N-(1-deoxy-d-fructos-1-yl) fumonisin B2 (NDfrc-FB2) and N-(1-deoxy-d-fructos-1-yl) fumonisin B3 (NDfrc-FB3) respectively, based on the accurate mass measurements of characteristic ions and fragmentation patterns using high-resolution liquid chromatography-Orbitrap mass spectrometry (LC-Orbitrap MS) analysis. Treatment on NDfrc-FB2 and NDfrc-FB3 with the o-phthalaldehyde (OPA) reagent also supported that d-glucose binding to FB2 and FB3 molecules occurred to their primary amine residues. View Full-Text
Keywords: LC-Orbitrap MS; fumonisin; Fusarium; corn; N-(1-deoxy-d-fructos-1-yl) fumonisin LC-Orbitrap MS; fumonisin; Fusarium; corn; N-(1-deoxy-d-fructos-1-yl) fumonisin
Show Figures

Graphical abstract

MDPI and ACS Style

Matsuo, Y.; Takahara, K.; Sago, Y.; Kushiro, M.; Nagashima, H.; Nakagawa, H. Detection of N-(1-deoxy-d-fructos-1-yl) Fumonisins B2 and B3 in Corn by High-Resolution LC-Orbitrap MS. Toxins 2015, 7, 3700-3714.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop