Bee Venom Protects against Rotenone-Induced Cell Death in NSC34 Motor Neuron Cells
AbstractRotenone, an inhibitor of mitochondrial complex I of the mitochondrial respiratory chain, is known to elevate mitochondrial reactive oxygen species and induce apoptosis via activation of the caspase-3 pathway. Bee venom (BV) extracted from honey bees has been widely used in oriental medicine and contains melittin, apamin, adolapin, mast cell-degranulating peptide, and phospholipase A2. In this study, we tested the effects of BV on neuronal cell death by examining rotenone-induced mitochondrial dysfunction. NSC34 motor neuron cells were pretreated with 2.5 μg/mL BV and stimulated with 10 μM rotenone to induce cell toxicity. We assessed cell death by Western blotting using specific antibodies, such as phospho-ERK1/2, phospho-JNK, and cleaved capase-3 and performed an MTT assay for evaluation of cell death and mitochondria staining. Pretreatment with 2.5 μg/mL BV had a neuroprotective effect against 10 μM rotenone-induced cell death in NSC34 motor neuron cells. Pre-treatment with BV significantly enhanced cell viability and ameliorated mitochondrial impairment in rotenone-treated cellular model. Moreover, BV treatment inhibited the activation of JNK signaling and cleaved caspase-3 related to cell death and increased ERK phosphorylation involved in cell survival in rotenone-treated NSC34 motor neuron cells. Taken together, we suggest that BV treatment can be useful for protection of neurons against oxidative stress or neurotoxin-induced cell death. View Full-Text
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Jung, S.Y.; Lee, K.-W.; Choi, S.-M.; Yang, E.J. Bee Venom Protects against Rotenone-Induced Cell Death in NSC34 Motor Neuron Cells. Toxins 2015, 7, 3715-3726.
Jung SY, Lee K-W, Choi S-M, Yang EJ. Bee Venom Protects against Rotenone-Induced Cell Death in NSC34 Motor Neuron Cells. Toxins. 2015; 7(9):3715-3726.Chicago/Turabian Style
Jung, So Y.; Lee, Kang-Woo; Choi, Sun-Mi; Yang, Eun J. 2015. "Bee Venom Protects against Rotenone-Induced Cell Death in NSC34 Motor Neuron Cells." Toxins 7, no. 9: 3715-3726.