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Open AccessArticle

Detection of Shiga Toxins by Lateral Flow Assay

1
Produce Safety & Microbiology Research Unit, Agricultural Research Service, U.S. Department of Agriculture, 800 Buchanan St, Albany, CA 94710, USA
2
Foodborne Toxin Detection and Prevention Research Unit, Agricultural Research Service, U.S. Department of Agriculture, 800 Buchanan St, Albany, CA 94710, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Gerald B. Koudelka
Toxins 2015, 7(4), 1163-1173; https://doi.org/10.3390/toxins7041163
Received: 4 March 2015 / Revised: 26 March 2015 / Accepted: 30 March 2015 / Published: 3 April 2015
(This article belongs to the Collection Shiga Toxins)
Shiga toxin-producing Escherichia coli (STEC) produce shiga toxins (Stxs) that can cause human disease and death. The contamination of food products with STEC represents a food safety problem that necessitates rapid and effective detection strategies to mitigate risk. In this manuscript, we report the development of a colorimetric lateral flow assay (LFA) for the rapid detection of Stxs in <10 min using a pair of monoclonal antibodies that bind epitopes common to Stx1 and six Stx2 variants. This LFA provides a rapid and sensitive test for the detection of Stxs directly from STEC culture supernatants or at risk food samples with a 0.1 ng/mL limit of detection (LOD) for Stx2a. This Stx LFA is applicable for use in the rapid evaluation of Stx production from cultured E. coli strains or as a tool to augment current methods as part of food safety testing. View Full-Text
Keywords: shiga toxin; Stx; toxin; STEC; E. coli O157; immunoassay; lateral flow assay; food safety shiga toxin; Stx; toxin; STEC; E. coli O157; immunoassay; lateral flow assay; food safety
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Ching, K.H.; He, X.; Stanker, L.H.; Lin, A.V.; McGarvey, J.A.; Hnasko, R. Detection of Shiga Toxins by Lateral Flow Assay. Toxins 2015, 7, 1163-1173.

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