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A Proteomics and Transcriptomics Investigation of the Venom from the Barychelid Spider Trittame loki (Brush-Foot Trapdoor)
Venom Evolution Lab, School of Biological Sciences, University of Queensland, St. Lucia, Queensland 4072, Australia
Institute for Molecular Bioscience, University of Queensland, St. Lucia, Queensland 4072, Australia
CIMAR/CIIMAR, Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Rua dos Bragas 177, Porto 4050-123, Portugal
Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, Porto 4169-007, Portugal
Centre for Advanced Imaging, University of Queensland, St. Lucia, Queensland 4072, Australia
HEJ Research Institute of Chemistry, International Centre for Chemical and Biological Sciences (ICCBS), University of Karachi, Karachi-75270, Pakistan
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 24 October 2013; in revised form: 29 November 2013 / Accepted: 9 December 2013 / Published: 13 December 2013
Abstract: Although known for their potent venom and ability to prey upon both invertebrate and vertebrate species, the Barychelidae spider family has been entirely neglected by toxinologists. In striking contrast, the sister family Theraphosidae (commonly known as tarantulas), which last shared a most recent common ancestor with Barychelidae over 200 million years ago, has received much attention, accounting for 25% of all the described spider toxins while representing only 2% of all spider species. In this study, we evaluated for the first time the venom arsenal of a barychelid spider, Trittame loki, using transcriptomic, proteomic, and bioinformatic methods. The venom was revealed to be dominated by extremely diverse inhibitor cystine knot (ICK)/knottin peptides, accounting for 42 of the 46 full-length toxin precursors recovered in the transcriptomic sequencing. In addition to documenting differential rates of evolution adopted by different ICK/knottin toxin lineages, we discovered homologues with completely novel cysteine skeletal architecture. Moreover, acetylcholinesterase and neprilysin were revealed for the first time as part of the spider-venom arsenal and CAP (CRiSP/Allergen/PR-1) were identified for the first time in mygalomorph spider venoms. These results not only highlight the extent of venom diversification in this neglected ancient spider lineage, but also reinforce the idea that unique venomous lineages are rich pools of novel biomolecules that may have significant applied uses as therapeutics and/or insecticides.
Keywords: venom; spider; mygalomorph; toxin; evolution
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Undheim, E.A.B.; Sunagar, K.; Herzig, V.; Kely, L.; Low, D.H.W.; Jackson, T.N.W.; Jones, A.; Kurniawan, N.; King, G.F.; Ali, S.A.; Antunes, A.; Ruder, T.; Fry, B.G. A Proteomics and Transcriptomics Investigation of the Venom from the Barychelid Spider Trittame loki (Brush-Foot Trapdoor). Toxins 2013, 5, 2488-2503.
Undheim EAB, Sunagar K, Herzig V, Kely L, Low DHW, Jackson TNW, Jones A, Kurniawan N, King GF, Ali SA, Antunes A, Ruder T, Fry BG. A Proteomics and Transcriptomics Investigation of the Venom from the Barychelid Spider Trittame loki (Brush-Foot Trapdoor). Toxins. 2013; 5(12):2488-2503.
Undheim, Eivind A.B.; Sunagar, Kartik; Herzig, Volker; Kely, Laurence; Low, Dolyce H.W.; Jackson, Timothy N.W.; Jones, Alun; Kurniawan, Nyoman; King, Glenn F.; Ali, Syed A.; Antunes, Agostino; Ruder, Tim; Fry, Bryan G. 2013. "A Proteomics and Transcriptomics Investigation of the Venom from the Barychelid Spider Trittame loki (Brush-Foot Trapdoor)." Toxins 5, no. 12: 2488-2503.