Effect of Dietary Difructose Anhydride III Supplementation on the Metabolic Profile of Japanese Black Breeding Herds with Low-Level Chronic Exposure to Zearalenone in the Dietary Feed

Priyo, Topas Wicaksono; Sasazaki, Naoya; Toda, Katsuki; Hasunuma, Hiroshi; Matsumoto, Daisaku; Kokushi, Emiko; Uno, Seiichi; Yamato, Osamu; Obi, Takeshi; Shinya, Urara; Widodo, Oky Setyo; Taura, Yasuho; Ono, Tetsushi; Taniguchi, Masayasu; Takagi, Mitsuhiro

doi:10.3390/toxins17080409

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Effect of Dietary Difructose Anhydride III Supplementation on the Metabolic Profile of Japanese Black Breeding Herds with Low-Level Chronic Exposure to Zearalenone in the Dietary Feed

by

Topas Wicaksono Priyo, Jr.

^1,2,3

,

Naoya Sasazaki

⁴,

Katsuki Toda

⁴,

Hiroshi Hasunuma

⁴,

Daisaku Matsumoto

⁴,

Emiko Kokushi

⁵,

Seiichi Uno

⁵

,

Osamu Yamato

^3,6

,

Takeshi Obi

⁶,

Urara Shinya

⁷,

Oky Setyo Widodo

⁸

,

Yasuho Taura

⁹,

Tetsushi Ono

⁹,

Masayasu Taniguchi

^1,9

and

Mitsuhiro Takagi

^1,8,9,*

¹

Joint Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 753-8515, Japan

²

Department of Reproduction and Obstetrics, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia

³

Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753-8515, Japan

⁴

Shepherd Central Livestock Clinic, Kagoshima 899-1611, Japan

⁵

Faculty of Fisheries, Kagoshima University, Kagoshima 890-0056, Japan

⁶

Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan

⁷

Soo Agricultural Mutual Aid Association, Kagoshima 899-8212, Japan

⁸

Division of Animal Husbandry, Faculty of Veterinary Medicine, Airlangga University, Surabaya 60115, Indonesia

⁹

Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753-8515, Japan

^*

Author to whom correspondence should be addressed.

Toxins 2025, 17(8), 409; https://doi.org/10.3390/toxins17080409 (registering DOI)

Submission received: 23 June 2025 / Revised: 2 August 2025 / Accepted: 11 August 2025 / Published: 14 August 2025

(This article belongs to the Special Issue Occurrence, Toxicity, Metabolism, Analysis and Control of Mycotoxins)

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Abstract

Mycotoxin contamination in animal feed can cause acute or chronic adverse effects on growth, productivity, and immune function in livestock. This study aimed to evaluate the impact of difructose anhydride III (DFA III) supplementation on serum biochemical parameters and intestinal environment in Japanese Black (JB) breeding cows under low-level chronic dietary exposure to zearalenone (ZEN). Using urinary ZEN concentration as an indicator of exposure, 25 JB cows were selected from a breeding farm with confirmed natural feed contamination. Blood samples were collected before DFA III supplementation (day 0), and on days 20 and 40 post-supplementation. Serum biochemical parameters and short-chain fatty acid concentrations were measured. During the studies, dietary ZEN concentration increased, yet improvements were observed in liver function, nutritional status, immune response, and inflammatory markers. Notably, serum butyrate concentration significantly increased following DFA III administration. These findings suggest that DFA III may positively influence intestinal microflora and enhance intestinal barrier function, which could contribute to improved health and nutritional status in cattle exposed to low-level chronic dietary ZEN contamination. DFA III supplementation may represent a promising strategy for mitigating the effects of low-level mycotoxin exposure in livestock production systems.

Keywords: Japanese Black cattle; difructose anhydride III; metabolic profile; zearalenone; intestinal barrier function

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MDPI and ACS Style

Priyo, T.W., Jr.; Sasazaki, N.; Toda, K.; Hasunuma, H.; Matsumoto, D.; Kokushi, E.; Uno, S.; Yamato, O.; Obi, T.; Shinya, U.; et al. Effect of Dietary Difructose Anhydride III Supplementation on the Metabolic Profile of Japanese Black Breeding Herds with Low-Level Chronic Exposure to Zearalenone in the Dietary Feed. Toxins 2025, 17, 409. https://doi.org/10.3390/toxins17080409

AMA Style

Priyo TW Jr., Sasazaki N, Toda K, Hasunuma H, Matsumoto D, Kokushi E, Uno S, Yamato O, Obi T, Shinya U, et al. Effect of Dietary Difructose Anhydride III Supplementation on the Metabolic Profile of Japanese Black Breeding Herds with Low-Level Chronic Exposure to Zearalenone in the Dietary Feed. Toxins. 2025; 17(8):409. https://doi.org/10.3390/toxins17080409

Chicago/Turabian Style

Priyo, Topas Wicaksono, Jr., Naoya Sasazaki, Katsuki Toda, Hiroshi Hasunuma, Daisaku Matsumoto, Emiko Kokushi, Seiichi Uno, Osamu Yamato, Takeshi Obi, Urara Shinya, and et al. 2025. "Effect of Dietary Difructose Anhydride III Supplementation on the Metabolic Profile of Japanese Black Breeding Herds with Low-Level Chronic Exposure to Zearalenone in the Dietary Feed" Toxins 17, no. 8: 409. https://doi.org/10.3390/toxins17080409

APA Style

Priyo, T. W., Jr., Sasazaki, N., Toda, K., Hasunuma, H., Matsumoto, D., Kokushi, E., Uno, S., Yamato, O., Obi, T., Shinya, U., Widodo, O. S., Taura, Y., Ono, T., Taniguchi, M., & Takagi, M. (2025). Effect of Dietary Difructose Anhydride III Supplementation on the Metabolic Profile of Japanese Black Breeding Herds with Low-Level Chronic Exposure to Zearalenone in the Dietary Feed. Toxins, 17(8), 409. https://doi.org/10.3390/toxins17080409

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Effect of Dietary Difructose Anhydride III Supplementation on the Metabolic Profile of Japanese Black Breeding Herds with Low-Level Chronic Exposure to Zearalenone in the Dietary Feed

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