Isolation and Aflatoxin B1-Degradation Characteristics of a Microbacterium proteolyticum B204 Strain from Bovine Faeces
Abstract
:1. Introduction
2. Results
2.1. Isolation, Identification and Characterization of AFB1 Degradation Bacterial Strains
2.2. Effects of Incubation pH and Temperature on AFB1 Degradation by M. proteolyticum B204
2.3. AFB1 Degradation by Cell-Free Culture Supernatant and Cell Extracts of M. proteolyticum B204
2.4. Effects of Heat Treatment, SDS, Proteinase K and EDTA on AFB1 Degradation by M. proteolyticum B204 Cell Culture Supernatant
2.5. Cytotoxicity Analysis of M. proteolyticum B204
2.6. Application on Food Matrices of AFB1 Detoxification by M. proteolyticum B204
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Chemical and Medium
5.2. Two-Round Screening and Isolation of AFB1 Degradation Bacterium
5.3. Analysis of AFB1 Degradation Products Using HPLC
area in experimental group)/AFB1 peak area in control group × 100%
5.4. Identification of AFB1 Degradation Bacterium
5.5. Exploration of Culture Conditions by M. proteolyticum B204 on AFB1 Degradation
5.6. AFB1 Degradation by Cell-Free Culture Supernatant, Cell-Free Extracts and Cells
5.7. Effects of Heat Treatment, SDS, Proteinase and EDTA on AFB1 Degradation by Cell-Free Culture Supernatant
5.8. Cytotoxicity Analysis by MTT Assay
5.9. Application of AFB1 Detoxification by M. proteolyticum B204 to Food Matrices
5.10. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Yan, Y.; Zhang, X.; Chen, H.; Huang, W.; Jiang, H.; Wang, C.; Xiao, Z.; Zhang, Y.; Xu, J. Isolation and Aflatoxin B1-Degradation Characteristics of a Microbacterium proteolyticum B204 Strain from Bovine Faeces. Toxins 2022, 14, 525. https://doi.org/10.3390/toxins14080525
Yan Y, Zhang X, Chen H, Huang W, Jiang H, Wang C, Xiao Z, Zhang Y, Xu J. Isolation and Aflatoxin B1-Degradation Characteristics of a Microbacterium proteolyticum B204 Strain from Bovine Faeces. Toxins. 2022; 14(8):525. https://doi.org/10.3390/toxins14080525
Chicago/Turabian StyleYan, Yi, Xinyue Zhang, Haiyan Chen, Wenmin Huang, Hongnian Jiang, Chulun Wang, Zhuang Xiao, Yuyu Zhang, and Jialiang Xu. 2022. "Isolation and Aflatoxin B1-Degradation Characteristics of a Microbacterium proteolyticum B204 Strain from Bovine Faeces" Toxins 14, no. 8: 525. https://doi.org/10.3390/toxins14080525
APA StyleYan, Y., Zhang, X., Chen, H., Huang, W., Jiang, H., Wang, C., Xiao, Z., Zhang, Y., & Xu, J. (2022). Isolation and Aflatoxin B1-Degradation Characteristics of a Microbacterium proteolyticum B204 Strain from Bovine Faeces. Toxins, 14(8), 525. https://doi.org/10.3390/toxins14080525