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Article

Experimental Evidence of Ciguatoxin Accumulation and Depuration in Carnivorous Lionfish

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Center for Marine Studies, Federal University of Paraná. Av. Beira-mar, s/n, Pontal do Paraná P.O. Box 61, Brazil
2
Université Côte d’Azur, CNRS, ECOSEAS, UMR7035, Parc Valrose, CEDEX 2, 06103 Nice, France
3
Environment Laboratories, Department of Nuclear Science and Application, International Atomic Energy Agency, 4 Quai Antoine 1er, 98000 Monaco, Monaco
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Laboratory of Marine Biotoxins, Institut Louis Malardé, UMR EIO (IFREMER, IRD, ILM, UPF), P.O. Box 30 Papeete, Tahiti, French Polynesia
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MMS Laboratory (EA 2160), Sciences and Techniques Faculty, Le Mans University, Avenue Olivier Messiaen, 72085 Le Mans, France
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Visiting Scientist Ifremer, Laboratoire Phycotoxines, Rue de I’lle d’Yeu, 44311 Nantes, France
*
Authors to whom correspondence should be addressed.
Toxins 2021, 13(8), 564; https://doi.org/10.3390/toxins13080564
Received: 23 June 2021 / Revised: 2 August 2021 / Accepted: 3 August 2021 / Published: 11 August 2021
(This article belongs to the Special Issue Ciguatoxins)
Ciguatera poisoning is a food intoxication associated with the consumption of fish or shellfish contaminated, through trophic transfer, with ciguatoxins (CTXs). In this study, we developed an experimental model to assess the trophic transfer of CTXs from herbivorous parrotfish, Chlorurus microrhinos, to carnivorous lionfish, Pterois volitans. During a 6-week period, juvenile lionfish were fed naturally contaminated parrotfish fillets at a daily dose of 0.11 or 0.035 ng CTX3C equiv. g−1, as measured by the radioligand-receptor binding assay (r-RBA) or neuroblastoma cell-based assay (CBA-N2a), respectively. During an additional 6-week depuration period, the remaining fish were fed a CTX-free diet. Using r-RBA, no CTXs were detectable in muscular tissues, whereas CTXs were measured in the livers of two out of nine fish sampled during exposure, and in four out of eight fish sampled during depuration. Timepoint pooled liver samples, as analyzed by CBA-N2a, confirmed the accumulation of CTXs in liver tissues, reaching 0.89 ng CTX3C equiv. g−1 after 41 days of exposure, followed by slow toxin elimination, with 0.37 ng CTX3C equiv. g−1 measured after the 6-week depuration. These preliminary results, which need to be pursued in adult lionfish, strengthen our knowledge on CTX transfer and kinetics along the food web. View Full-Text
Keywords: ciguatoxins; experimental exposure; lionfish; trophic transfer; toxin accumulation; ciguatera poisoning ciguatoxins; experimental exposure; lionfish; trophic transfer; toxin accumulation; ciguatera poisoning
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MDPI and ACS Style

Leite, I.d.P.; Sdiri, K.; Taylor, A.; Viallon, J.; Gharbia, H.B.; Mafra Júnior, L.L.; Swarzenski, P.; Oberhaensli, F.; Darius, H.T.; Chinain, M.; Bottein, M.-Y.D. Experimental Evidence of Ciguatoxin Accumulation and Depuration in Carnivorous Lionfish. Toxins 2021, 13, 564. https://doi.org/10.3390/toxins13080564

AMA Style

Leite IdP, Sdiri K, Taylor A, Viallon J, Gharbia HB, Mafra Júnior LL, Swarzenski P, Oberhaensli F, Darius HT, Chinain M, Bottein M-YD. Experimental Evidence of Ciguatoxin Accumulation and Depuration in Carnivorous Lionfish. Toxins. 2021; 13(8):564. https://doi.org/10.3390/toxins13080564

Chicago/Turabian Style

Leite, Isabel do Prado, Khalil Sdiri, Angus Taylor, Jérôme Viallon, Hela Ben Gharbia, Luiz Laureno Mafra Júnior, Peter Swarzenski, François Oberhaensli, Hélène Taiana Darius, Mireille Chinain, and Marie-Yasmine Dechraoui Bottein. 2021. "Experimental Evidence of Ciguatoxin Accumulation and Depuration in Carnivorous Lionfish" Toxins 13, no. 8: 564. https://doi.org/10.3390/toxins13080564

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