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The Potential of Peroxidases Extracted from the Spent Mushroom (Flammulina velutipes) Substrate Significantly Degrade Mycotoxin Deoxynivalenol
Open AccessArticle

Deoxynivalenol Induces Caspase-8-Mediated Apoptosis through the Mitochondrial Pathway in Hippocampal Nerve Cells of Piglet

College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
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Toxins 2021, 13(2), 73; https://doi.org/10.3390/toxins13020073
Received: 3 December 2020 / Revised: 12 January 2021 / Accepted: 15 January 2021 / Published: 20 January 2021
(This article belongs to the Section Mycotoxins)
Deoxynivalenol (DON) is a common trichothecene mycotoxin found worldwide. DON has broad toxicity towards animals and humans. However, the mechanism of DON-induced neurotoxicity in vitro has not been fully understood. This study investigated the hypothesis that DON toxicity in neurons occurs via the mitochondrial apoptotic pathway. Using piglet hippocampal nerve cells (PHNCs), we evaluated the effects of different concentrations of DON on typical indicators of apoptosis. The obtained results demonstrated that DON treatment inhibited PHNC proliferation and led to morphological, biochemical, and transcriptional changes consistent with apoptosis, including decreased mitochondrial membrane potential, mitochondrial release of cytochrome C (CYCS) and apoptosis inducing factor (AIF), and increased abundance of active cleaved-caspase-9 and cleaved-caspase-3. Increasing concentrations of DON led to decreased B-cell lymphoma-2 (Bcl-2) expression and increased expression of BCL2-associated X (Bax) and B-cell lymphoma-2 homology 3 interacting domain death agonist (Bid), which in turn increased transcriptional activity of the transcription factors AIF and P53 (a tumor suppressor gene, promotes apoptosis). The addition of a caspase-8 inhibitor abrogated these effects. These results reveal that DON induces apoptosis in PHNCs via the mitochondrial apoptosis pathway, and caspase-8 is shown to play an important role during apoptosis regulation. View Full-Text
Keywords: deoxynivalenol; apoptosis; mitochondrial pathway; piglet hippocampal nerve cells deoxynivalenol; apoptosis; mitochondrial pathway; piglet hippocampal nerve cells
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MDPI and ACS Style

Cao, L.; Jiang, Y.; Zhu, L.; Xu, W.; Chu, X.; Zhang, Y.; Rahman, S.U.; Feng, S.; Li, Y.; Wu, J.; Wang, X. Deoxynivalenol Induces Caspase-8-Mediated Apoptosis through the Mitochondrial Pathway in Hippocampal Nerve Cells of Piglet. Toxins 2021, 13, 73. https://doi.org/10.3390/toxins13020073

AMA Style

Cao L, Jiang Y, Zhu L, Xu W, Chu X, Zhang Y, Rahman SU, Feng S, Li Y, Wu J, Wang X. Deoxynivalenol Induces Caspase-8-Mediated Apoptosis through the Mitochondrial Pathway in Hippocampal Nerve Cells of Piglet. Toxins. 2021; 13(2):73. https://doi.org/10.3390/toxins13020073

Chicago/Turabian Style

Cao, Li; Jiang, Yunjing; Zhu, Lei; Xu, Wei; Chu, Xiaoyan; Zhang, Yafei; Rahman, Sajid U.; Feng, Shibin; Li, Yu; Wu, Jinjie; Wang, Xichun. 2021. "Deoxynivalenol Induces Caspase-8-Mediated Apoptosis through the Mitochondrial Pathway in Hippocampal Nerve Cells of Piglet" Toxins 13, no. 2: 73. https://doi.org/10.3390/toxins13020073

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