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Open AccessArticle

The Effectiveness of Dietary Byproduct Antioxidants on Induced CYP Genes Expression and Histological Alteration in Piglets Liver and Kidney Fed with Aflatoxin B1 and Ochratoxin A

1
Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, Splaiul Independentei No. 91–95, 050095 Bucharest, Romania
2
Laboratory of Animal Biology, National Institute for Research and Development for Biology and Animal Nutrition, Calea Bucuresti No. 1, Balotesti, 077015 Ilfov, Romania
3
Raluca Ripan Institute for Research in Chemistry, Babeş Bolyai University, 30 Fântânele Street, 400294 Cluj-Napoca, Romania
4
“Aurel Ardelean” Institute of Life Sciences, Vasile Godis Western University of Arad, Rebreanu 86, 310414 Arad, Romania
*
Authors to whom correspondence should be addressed.
Toxins 2021, 13(2), 148; https://doi.org/10.3390/toxins13020148
Received: 11 January 2021 / Revised: 12 February 2021 / Accepted: 13 February 2021 / Published: 15 February 2021
The purpose of this study was to investigate the potential of a byproduct mixture derived from grapeseed and sea buckthorn oil industry to mitigate the harmful damage produced by ochratoxin A and aflatoxin B1 at hepatic and renal level in piglets after weaning. Forty cross-bred TOPIGS-40 hybrid piglets after weaning were assigned to three experimental groups (E1, E2, E3) and one control group (C), and fed with experimental diets for 30 days. The basal diet was served as a control and contained normal compound feed for starter piglets without mycotoxins. The experimental groups were fed as follows: E1—basal diet plus a mixture (1:1) of two byproducts (grapeseed and sea buckthorn meal); E2—the basal diet experimentally contaminated with mycotoxins (479 ppb OTA and 62ppb AFB1); and E3—basal diet containing 5% of the mixture (1:1) of grapeseed and sea buckthorn meal and contaminated with the mix of OTA and AFB1. After 4 weeks, the animals were slaughtered, and tissue samples were taken from liver and kidney in order to perform gene expression and histological analysis. The gene expression analysis showed that when weaned piglets were fed with contaminated diet, the expression of most analyzed genes was downregulated. Among the CYP450 family, CYP1A2 was the gene with the highest downregulation. According to these results, in liver, we found that mycotoxins induced histomorphological alterations in liver and kidney and had an effect on the expression level of CYP1A2, CYP2A19, CYP2E1, and CYP3A29, but we did not detect important changes in the expression level of CY4A24, MRP2 and GSTA1 genes. View Full-Text
Keywords: piglets; antioxidant effect; feed additives; mycotoxins; CYPs gene expression piglets; antioxidant effect; feed additives; mycotoxins; CYPs gene expression
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MDPI and ACS Style

Popescu, R.G.; Bulgaru, C.; Untea, A.; Vlassa, M.; Filip, M.; Hermenean, A.; Marin, D.; Țăranu, I.; Georgescu, S.E.; Dinischiotu, A. The Effectiveness of Dietary Byproduct Antioxidants on Induced CYP Genes Expression and Histological Alteration in Piglets Liver and Kidney Fed with Aflatoxin B1 and Ochratoxin A. Toxins 2021, 13, 148. https://doi.org/10.3390/toxins13020148

AMA Style

Popescu RG, Bulgaru C, Untea A, Vlassa M, Filip M, Hermenean A, Marin D, Țăranu I, Georgescu SE, Dinischiotu A. The Effectiveness of Dietary Byproduct Antioxidants on Induced CYP Genes Expression and Histological Alteration in Piglets Liver and Kidney Fed with Aflatoxin B1 and Ochratoxin A. Toxins. 2021; 13(2):148. https://doi.org/10.3390/toxins13020148

Chicago/Turabian Style

Popescu, Roua G.; Bulgaru, Cristina; Untea, Arabela; Vlassa, Mihaela; Filip, Miuta; Hermenean, Anca; Marin, Daniela; Țăranu, Ionelia; Georgescu, Sergiu E.; Dinischiotu, Anca. 2021. "The Effectiveness of Dietary Byproduct Antioxidants on Induced CYP Genes Expression and Histological Alteration in Piglets Liver and Kidney Fed with Aflatoxin B1 and Ochratoxin A" Toxins 13, no. 2: 148. https://doi.org/10.3390/toxins13020148

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