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Open AccessArticle

Algicidal Molecular Mechanism and Toxicological Degradation of Microcystis aeruginosa by White-Rot Fungi

by Guoming Zeng 1,2, Pei Gao 1,2, Jiale Wang 1,2, Jinxi Zhang 1,2, Maolan Zhang 1,2,* and Da Sun 3,*
1
Chongqing Engineering Laboratory of Nano/Micro Biological Medicine Detection Technology, School of Architecture and Engineering, Chongqing University of Science and Technology, Chongqing 401331, China
2
China Metallurgical Construction Engineering Group Co., Ltd., Chongqing 643000, China
3
Institute of Life Sciences & Biomedicine Collaborative Innovation Center, Wenzhou University, Wenzhou 325035, China
*
Authors to whom correspondence should be addressed.
Toxins 2020, 12(6), 406; https://doi.org/10.3390/toxins12060406
Received: 4 May 2020 / Revised: 9 June 2020 / Accepted: 18 June 2020 / Published: 19 June 2020
(This article belongs to the Special Issue Isolation and Characterization of Marine Toxins)
Current research on the inhibition of Microcystis aeruginosa growth is primarily focused on algae-lysing bacteria, and few studies have investigated the inhibitory mechanisms by which fungi affect it at the molecular level. A comparative analysis of the effects of Phanerochaete chrysosporium on the expression of the algal cell antioxidant protease synthesis gene prx, the biological macromolecule damage and repair genes recA, grpE, and fabZ, and the photosynthesis system-related genes psaB, psbD1 and rbcL, as well as genes for algal toxin synthesis mcyB, were performed to elucidate the molecular mechanism of Phanerochaete chrysosporium against Microcystis aeruginosa cells. RT-qPCR technology was used to study the molecular mechanism of algal cell inhibition by Phanerochaete chrysosporium liquid containing metabolites of Phanerochaete chrysosporium, Phanerochaete chrysosporium supernatant and Phanerochaete chrysosporium inactivated via high temperature sterilization at the gene expression level. Compared with the control, the chlorophyll-a contents dropped, and the recA, grpE, fabZ, and prx increased, but the psaB, psbD1, rbcL and mcyB showed that they were significantly reduced, which indicated that Phanerochaete chrysosporium can not only effectively destroy algal cells, but they may also reduce the expression of the Microcystis aeruginosa toxin gene and significantly block the metabolic system underlying the growth of algal cells and the synthesis of microcystins. View Full-Text
Keywords: white-rot fungi; Microcystis aeruginosa; molecular mechanism; microcystins white-rot fungi; Microcystis aeruginosa; molecular mechanism; microcystins
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Zeng, G.; Gao, P.; Wang, J.; Zhang, J.; Zhang, M.; Sun, D. Algicidal Molecular Mechanism and Toxicological Degradation of Microcystis aeruginosa by White-Rot Fungi. Toxins 2020, 12, 406.

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