Oligomer Formation and Insecticidal Activity of Bacillus thuringiensis Vip3Aa Toxin

Shao, Ensi; Zhang, Aishan; Yan, Yaqi; Wang, Yaomin; Jia, Xinyi; Sha, Li; Guan, Xiong; Wang, Ping; Huang, Zhipeng

doi:10.3390/toxins12040274

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Oligomer Formation and Insecticidal Activity of Bacillus thuringiensis Vip3Aa Toxin

by

Ensi Shao

^1,†

,

Aishan Zhang

^1,†,

Yaqi Yan

¹,

Yaomin Wang

¹,

Xinyi Jia

¹,

Li Sha

¹,

Xiong Guan

²

,

Ping Wang

^3,* and

Zhipeng Huang

^1,*

¹

China National Engineering Research Center of JUNCAO Technology, College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China

²

Key Laboratory of Biopesticide and Chemical Biology (Ministry of Education), College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China

³

Department of Entomology, Cornell University, Geneva, NY 14456, USA

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Toxins 2020, 12(4), 274; https://doi.org/10.3390/toxins12040274

Submission received: 19 March 2020 / Revised: 17 April 2020 / Accepted: 21 April 2020 / Published: 23 April 2020

(This article belongs to the Special Issue Bacillus thuringiensis Toxins: Functional Characterization and Mechanism of Action)

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Abstract

Bacillus thuringiensis (Bt) Vip3A proteins are important insecticidal proteins used for control of lepidopteran insects. However, the mode of action of Vip3A toxin is still unclear. In this study, the amino acid residue S164 in Vip3Aa was identified to be critical for the toxicity in Spodoptera litura. Results from substitution mutations of the S164 indicate that the insecticidal activity of Vip3Aa correlated with the formation of a >240 kDa complex of the toxin upon proteolytic activation. The >240 kDa complex was found to be composed of the 19 kDa and the 65 kDa fragments of Vip3Aa. Substitution of the S164 in Vip3Aa protein with Ala or Pro resulted in loss of the >240 kDa complex and loss of toxicity in Spodoptera litura. In contrast, substitution of S164 with Thr did not affect the >240 kDa complex formation, and the toxicity of the mutant was only reduced by 35%. Therefore, the results from this study indicated that formation of the >240 kDa complex correlates with the toxicity of Vip3Aa in insects and the residue S164 is important for the formation of the complex.

Keywords: Bacillus thuringiensis; Vip3A; Spodoptera litura; site-directed mutagenesis

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MDPI and ACS Style

Shao, E.; Zhang, A.; Yan, Y.; Wang, Y.; Jia, X.; Sha, L.; Guan, X.; Wang, P.; Huang, Z. Oligomer Formation and Insecticidal Activity of Bacillus thuringiensis Vip3Aa Toxin. Toxins 2020, 12, 274. https://doi.org/10.3390/toxins12040274

AMA Style

Shao E, Zhang A, Yan Y, Wang Y, Jia X, Sha L, Guan X, Wang P, Huang Z. Oligomer Formation and Insecticidal Activity of Bacillus thuringiensis Vip3Aa Toxin. Toxins. 2020; 12(4):274. https://doi.org/10.3390/toxins12040274

Chicago/Turabian Style

Shao, Ensi, Aishan Zhang, Yaqi Yan, Yaomin Wang, Xinyi Jia, Li Sha, Xiong Guan, Ping Wang, and Zhipeng Huang. 2020. "Oligomer Formation and Insecticidal Activity of Bacillus thuringiensis Vip3Aa Toxin" Toxins 12, no. 4: 274. https://doi.org/10.3390/toxins12040274

APA Style

Shao, E., Zhang, A., Yan, Y., Wang, Y., Jia, X., Sha, L., Guan, X., Wang, P., & Huang, Z. (2020). Oligomer Formation and Insecticidal Activity of Bacillus thuringiensis Vip3Aa Toxin. Toxins, 12(4), 274. https://doi.org/10.3390/toxins12040274

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Oligomer Formation and Insecticidal Activity of Bacillus thuringiensis Vip3Aa Toxin

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