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Open AccessArticle

Investigation of In Vitro Endocrine Activities of Microcystis and Planktothrix Cyanobacterial Strains

1
Toxinology Research Group, Norwegian Veterinary Institute, Ullevålsveien 68, N-0454 Oslo, Norway
2
Department of Chemistry, University of Oslo, P.O. Box 1033, N-0315 Oslo, Norway
3
Institute for Global Food Security, School of Biological Sciences, Queen’s University Belfast, Belfast BT9 5DL, UK
*
Author to whom correspondence should be addressed.
Toxins 2020, 12(4), 228; https://doi.org/10.3390/toxins12040228
Received: 27 February 2020 / Revised: 29 March 2020 / Accepted: 1 April 2020 / Published: 4 April 2020
Cyanobacteria are cosmopolitan photosynthetic prokaryotes that can form dense accumulations in aquatic environments. They are able to produce many bioactive metabolites, some of which are potentially endocrine disrupting compounds, i.e., compounds that interfere with the hormonal systems of animals and humans. Endocrine disruptors represent potential risks to both environmental and human health, making them a global challenge. The aim of this study was to investigate the potential endocrine disrupting activities with emphasis on estrogenic effects of extracts from cultures of Microcystis or Planktothrix species. We also assessed the possible role of microcystins, some of the most studied cyanobacterial toxins, and thus included both microcystin-producing and non-producing strains. Extracts from 26 cyanobacterial cultures were initially screened in estrogen-, androgen-, and glucocorticoid-responsive reporter-gene assays (RGAs) in order to identify endocrine disruption at the level of nuclear receptor transcriptional activity. Extracts from selected strains were tested repeatedly in the estrogen-responsive RGAs, but the observed estrogen agonist and antagonist activity was minor and similar to that of the cyanobacteria growth medium control. We thus focused on another, non-receptor mediated mechanism of action, and studied the 17β-estradiol (natural estrogen hormone) biotransformation in human liver microsomes in the presence or absence of microcystin-LR (MC-LR), or an extract from the MC-LR producing M. aeruginosa PCC7806 strain. Our results show a modulating effect on the estradiol biotransformation. Thus, while 2-hydroxylation was significantly decreased following co-incubation of 17β-estradiol with MC-LR or M. aeruginosa PCC7806 extract, the relative concentration of estrone was increased. View Full-Text
Keywords: cyanobacteria; endocrine disruptor; estrogenic; microcystin; reporter-gene assay; Planktothrix; Microcystis cyanobacteria; endocrine disruptor; estrogenic; microcystin; reporter-gene assay; Planktothrix; Microcystis
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Mallia, V.; Ivanova, L.; Eriksen, G.S.; Harper, E.; Connolly, L.; Uhlig, S. Investigation of In Vitro Endocrine Activities of Microcystis and Planktothrix Cyanobacterial Strains. Toxins 2020, 12, 228.

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