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Open AccessArticle

Bacillus cereus Decreases NHE and CLO Exotoxin Synthesis to Maintain Appropriate Proteome Dynamics During Growth at Low Temperature

Avignon University, Biology Department, INRAE, UMR SQPOV, F-84914 Avignon, France
Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI, 30200 Bagnols-sur-Cèze, France
Author to whom correspondence should be addressed.
This author is no longer affiliated with this institution.
Toxins 2020, 12(10), 645;
Received: 31 July 2020 / Revised: 17 September 2020 / Accepted: 23 September 2020 / Published: 6 October 2020
(This article belongs to the Special Issue Advanced Proteomics as a Powerful Tool for Studying Bacterial Toxins)
Cellular proteomes and exoproteomes are dynamic, allowing pathogens to respond to environmental conditions to sustain growth and virulence. Bacillus cereus is an important food-borne pathogen causing intoxication via emetic toxin and/or multiple protein exotoxins. Here, we compared the dynamics of the cellular proteome and exoproteome of emetic B. cereus cells grown at low (16 °C) and high (30 °C) temperature. Tandem mass spectrometry (MS/MS)-based shotgun proteomics analysis identified 2063 cellular proteins and 900 extracellular proteins. Hierarchical clustering following principal component analysis indicated that in B. cereus the abundance of a subset of these proteins—including cold-stress responders, and exotoxins non-hemolytic enterotoxin (NHE) and hemolysin I (cereolysin O (CLO))—decreased at low temperature, and that this subset governs the dynamics of the cellular proteome. NHE, and to a lesser extent CLO, also contributed significantly to exoproteome dynamics; with decreased abundances in the low-temperature exoproteome, especially in late growth stages. Our data therefore indicate that B. cereus may reduce its production of secreted protein toxins to maintain appropriate proteome dynamics, perhaps using catabolite repression to conserve energy for growth in cold-stress conditions, at the expense of virulence. View Full-Text
Keywords: Bacillus cereus; shotgun proteomics; exotoxins; low-temperature Bacillus cereus; shotgun proteomics; exotoxins; low-temperature
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MDPI and ACS Style

Duport, C.; Rousset, L.; Alpha-Bazin, B.; Armengaud, J. Bacillus cereus Decreases NHE and CLO Exotoxin Synthesis to Maintain Appropriate Proteome Dynamics During Growth at Low Temperature. Toxins 2020, 12, 645.

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