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Validation of Predicted Virulence Factors in Listeria monocytogenes Identified Using Comparative Genomics

Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA
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Toxins 2019, 11(9), 508; https://doi.org/10.3390/toxins11090508
Received: 30 July 2019 / Revised: 14 August 2019 / Accepted: 24 August 2019 / Published: 30 August 2019
(This article belongs to the Special Issue Toxins and Virulence Factors of Listeria monocytogenes)
Listeria monocytogenes is an intracellular facultative pathogen that causes listeriosis, a foodborne zoonotic infection. There are differences in the pathogenic potential of L. monocytogenes subtypes and strains. Comparison of the genome sequences among L. monocytogenes pathogenic strains EGD-e and F2365 with nonpathogenic L. innocua CLIP1182 and L. monocytogenes strain HCC23 revealed a set of proteins that were present in pathogenic strains and had no orthologs among the nonpathogenic strains. Among the candidate virulence factors are five proteins: putrescine carbamoyltransferase; InlH/InlC2 family class 1 internalin; phosphotransferase system (PTS) fructose transporter subunit EIIC; putative transketolase; and transcription antiterminator BglG family. To determine if these proteins have a role in adherence and invasion of intestinal epithelial Caco-2 cells and/or contribute to virulence, five mutant strains were constructed. F2365ΔinlC2, F2365Δeiic, and F2365Δtkt exhibited a significant (p < 0.05) reduction in adhesion to Caco-2 cells compared to parent F2365 strain. The invasion of F2365ΔaguB, F2365ΔinlC2, and F2365ΔbglG decreased significantly (p < 0.05) compared with the parent strain. Bacterial loads in mouse liver and spleen infected by F2365 was significantly (p < 0.05) higher than it was for F2365ΔaguB, F2365ΔinlC2, F2365Δeiic, F2365Δtkt, and F2365ΔbglG strains. This study demonstrates that aguB, inlC2, eiic, tkt, and bglG play a role in L. monocytogenes pathogenicity. View Full-Text
Keywords: Listeria monocytogenes; adhesion; invasion; mice infection Listeria monocytogenes; adhesion; invasion; mice infection
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Abdelhamed, H.; Lawrence, M.L.; Ramachandran, R.; Karsi, A. Validation of Predicted Virulence Factors in Listeria monocytogenes Identified Using Comparative Genomics. Toxins 2019, 11, 508.

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