Synthesis and Characterization of Cry2Ab–AVM Bioconjugate: Enhanced Affinity to Binding Proteins and Insecticidal Activity
Abstract
:1. Introduction
2. Results
2.1. Preparation and Characterization of Cry2Ab–AVM
2.2. Cry2Ab–AVM Binds to PxCR10–11 at Higher Affinity Than Cry2Ab30
2.3. Insecticidal Toxicity Bioassay
2.4. Modeling and Docking Analysis
3. Discussion
4. Materials and Methods
4.1. Insects
4.2. Synthesis of 4”-O-Succinyl Avermectin
4.3. Preparation of Cry2Ab30
4.4. Preparation of Truncated Recombinant P. xylostella Cadherin (PxCR10–11)
4.5. General Procedure for Cry2Ab–AVM
4.6. Ligand Blot Assay
4.7. Biosensor-Binding Kinetics
4.8. Toxicity Assays
4.9. Transmission Electron Microscope Analysis
4.10. Homology Modeling and Docking
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Toxin | LC50 (μg/cm2) | 95% Confidence Interval | Slope | SE | Relative Potency a |
---|---|---|---|---|---|
Cry2Ab30 | 1.544 | 1.041–2.402 | 1.922 | 0.333 | 1 |
Cry2Ab–AVM | 0.010 | 0.006–0.016 | 1.792 | 0.356 | 154.4 |
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Pan, Z.-Z.; Xu, L.; Zheng, Y.-S.; Niu, L.-Y.; Liu, B.; Fu, N.-Y.; Shi, Y.; Chen, Q.-X.; Zhu, Y.-J.; Guan, X. Synthesis and Characterization of Cry2Ab–AVM Bioconjugate: Enhanced Affinity to Binding Proteins and Insecticidal Activity. Toxins 2019, 11, 497. https://doi.org/10.3390/toxins11090497
Pan Z-Z, Xu L, Zheng Y-S, Niu L-Y, Liu B, Fu N-Y, Shi Y, Chen Q-X, Zhu Y-J, Guan X. Synthesis and Characterization of Cry2Ab–AVM Bioconjugate: Enhanced Affinity to Binding Proteins and Insecticidal Activity. Toxins. 2019; 11(9):497. https://doi.org/10.3390/toxins11090497
Chicago/Turabian StylePan, Zhi-Zhen, Lian Xu, Yi-Shu Zheng, Li-Yang Niu, Bo Liu, Nan-Yan Fu, Yan Shi, Qing-Xi Chen, Yu-Jing Zhu, and Xiong Guan. 2019. "Synthesis and Characterization of Cry2Ab–AVM Bioconjugate: Enhanced Affinity to Binding Proteins and Insecticidal Activity" Toxins 11, no. 9: 497. https://doi.org/10.3390/toxins11090497