Clostridium botulinum and Clostridium perfringens Occurrence in Kazakh Honey Samples
Balgabay Maikanov 1, Raikhan Mustafina 1, Laura Auteleyeva 1
, Jan Wiśniewski 2, Krzysztof Anusz 2, Tomasz Grenda 3,*, Krzysztof Kwiatek 3, Magdalena Goldsztejn 3 and Magdalena Grabczak 3
, Jan Wiśniewski 2, Krzysztof Anusz 2, Tomasz Grenda 3,*, Krzysztof Kwiatek 3, Magdalena Goldsztejn 3 and Magdalena Grabczak 3
1
Department of Veterinary Sanitation, Faculty of Veterinary Medicine and Technology of Animal Husbandry, S. Seifullin Kazakh Agro Technical University, Zhenis Avenue 62, 010011 Astana, Republic of Kazakhstan
2
Departament of Food Hygiene and Public Health Protection, Faculty of Veterinary Medicine, Warsaw University of Life Sciences—SGGW (WULS-SGGW), ul.Nowoursynowska 159, 02-776 Warsaw, Poland
3
Department of Hygiene of Animal Feeding Stuffs, National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, Poland
*
Author to whom correspondence should be addressed.
Toxins 2019, 11(8), 472; https://doi.org/10.3390/toxins11080472
Received: 14 June 2019 / Revised: 8 August 2019 / Accepted: 9 August 2019 / Published: 13 August 2019
(This article belongs to the Special Issue New Challenges in Foodborne Botulism Outbreaks)
Abstract
The aim of this study was to assess occurrence of Clostridium botulinum and Clostridium perfringens in honey samples from Kazakhstan. Analyses were carried out using a set of PCR methods for identification of anaerobic bacteria, and detection of toxin genes of C. botulinum and C. perfringens. Among 197 samples, C. botulinum was noticed in only one (0.5%). The isolated strain of this pathogen showed the presence of the bont/A and ntnh genes. C. perfringens strains were isolated from 18 (9%) samples, and mPCR (multiplex PCR) analysis led to them all being classified as toxin type A with the ability to produce α toxin. Sequence analysis of 16S rDNA genes showed occurrence in 4 samples of other anaerobes related to C. botulinum, which were C. sporogenes and C. beijerinckii strains. C. botulinum prevalence in honey samples from Kazakhstan in comparison to the prevalence in samples collected from the other regions seems to be less. The highest prevalence of Clostridium sp. was noticed in the East Kazakhstan province. Our study is the first survey on BoNT-producing clostridia and C. perfringens prevalence in Kazakh honey. View Full-TextKeywords:
C. botulinum; C. perfringens; honey; Kazakhstan
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Maikanov, B.; Mustafina, R.; Auteleyeva, L.; Wiśniewski, J.; Anusz, K.; Grenda, T.; Kwiatek, K.; Goldsztejn, M.; Grabczak, M. Clostridium botulinum and Clostridium perfringens Occurrence in Kazakh Honey Samples. Toxins 2019, 11, 472.
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