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Insecticidal Activity of a Vip3Ab1 Chimera Is Conferred by Improved Protein Stability in the Midgut of Spodoptera eridania
Open AccessArticle

Modification of Vip3Ab1 C-Terminus Confers Broadened Plant Protection from Lepidopteran Pests

Corteva Agriscience, Indianapolis, IN 46268, USA
*
Authors to whom correspondence should be addressed.
Present address: Indiana Biosciences Research Institute, Indianapolis, IN 46202, USA.
Present address: GreenLight Biosciences, Durham, NC 27709, USA.
§
Present address: Genective Corporation, Weldon, IL 61882, USA.
Toxins 2019, 11(6), 316; https://doi.org/10.3390/toxins11060316
Received: 22 April 2019 / Revised: 22 May 2019 / Accepted: 30 May 2019 / Published: 3 June 2019
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Abstract

Vegetative insecticidal proteins (Vips) from Bacillus thuringiensis (Bt) are unique from crystal (Cry) proteins found in Bt parasporal inclusions as they are secreted during the bacterial vegetative growth phase and bind unique receptors to exert their insecticidal effects. We previously demonstrated that large modifications of the Vip3 C-terminus could redirect insecticidal spectrum but results in an unstable protein with no lethal activity. In the present work, we have generated a new Vip3 protein, Vip3Ab1-740, via modest modification of the Vip3Ab1 C-terminus. Vip3Ab1-740 is readily processed by midgut fluid enzymes and has lethal activity towards Spodoptera eridania, which is not observed with the Vip3Ab1 parent protein. Importantly, Vip3Ab1-740 does retain the lethal activity of Vip3Ab1 against other important lepidopteran pests. Furthermore, transgenic plants expressing Vip3Ab1-740 are protected against S. eridania, Spodoptera frugiperda, Helicoverpa zea, and Pseudoplusia includens. Thus, these studies demonstrate successful engineering of Vip3 proteins at the C-terminus to broaden insecticidal spectrum, which can be employed for functional expression in planta. View Full-Text
Keywords: vegetative insecticidal protein; Vip3; spodoptera; lepidoptera vegetative insecticidal protein; Vip3; spodoptera; lepidoptera
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Sopko, M.S.; Narva, K.E.; Bowling, A.J.; Pence, H.E.; Hasler, J.J.; Letherer, T.J.; Larsen, C.M.; Zack, M.D. Modification of Vip3Ab1 C-Terminus Confers Broadened Plant Protection from Lepidopteran Pests. Toxins 2019, 11, 316.

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