Clostridium perfringens Delta-Toxin Damages the Mouse Small Intestine
AbstractClostridium perfringens strains B and C cause fatal intestinal diseases in animals. The secreted pore-forming toxin delta-toxin is one of the virulence factors of the strains, but the mechanism of intestinal pathogenesis is unclear. Here, we investigated the effects of delta-toxin on the mouse ileal loop. Delta-toxin caused fluid accumulation and intestinal permeability to fluorescein isothiocyanate (FITC)-dextran in the mouse ileal loop in a dose- and time-dependent manner. Treatment with delta-toxin induced significant histological damage and shortening of villi. Delta-toxin activates a disintegrin and metalloprotease (ADAM) 10, leading to the cleavage of E-cadherin, the epithelial adherens junction protein, in human intestinal epithelial Caco-2 cells. In this study, E-cadherin immunostaining in mouse intestinal epithelial cells was almost undetectable 1 h after toxin treatment. ADAM10 inhibitor (GI254023X) blocked the toxin-induced fluid accumulation and E-cadherin loss in the mouse ileal loop. Delta-toxin stimulated the shedding of intestinal epithelial cells. The shedding cells showed the accumulation of E-cadherin in intracellular vesicles and the increased expression of active caspase-3. Our findings demonstrate that delta-toxin causes intestinal epithelial cell damage through the loss of E-cadherin cleaved by ADAM10. View Full-Text
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Seike, S.; Takehara, M.; Kobayashi, K.; Nagahama, M. Clostridium perfringens Delta-Toxin Damages the Mouse Small Intestine. Toxins 2019, 11, 232.
Seike S, Takehara M, Kobayashi K, Nagahama M. Clostridium perfringens Delta-Toxin Damages the Mouse Small Intestine. Toxins. 2019; 11(4):232.Chicago/Turabian Style
Seike, Soshi; Takehara, Masaya; Kobayashi, Keiko; Nagahama, Masahiro. 2019. "Clostridium perfringens Delta-Toxin Damages the Mouse Small Intestine." Toxins 11, no. 4: 232.
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