Next Article in Journal
Deoxynivalenol Induces Inflammatory Injury in IPEC-J2 Cells via NF-κB Signaling Pathway
Next Article in Special Issue
Alteration of Bumblebee Venom Composition toward Higher Elevation
Previous Article in Journal
The Cytotoxicity Effect of Resveratrol: Cell Cycle Arrest and Induced Apoptosis of Breast Cancer 4T1 Cells
Previous Article in Special Issue
Alternative mRNA Splicing in Three Venom Families Underlying a Possible Production of Divergent Venom Proteins of the Habu Snake, Protobothrops flavoviridis

The Peptide Venom Composition of the Fierce Stinging Ant Tetraponera aethiops (Formicidae: Pseudomyrmecinae)

EA-7417, Institut National Universitaire Champollion, Place de Verdun, 81012 Albi, France
Laboratory of Animal Biology and Physiology, Faculty of Science, University of Douala, P.O. Box 24157, Douala, Cameroon
Unité de Mathématique et Informatique Appliquées de Toulouse, Genotoul Bioinfo, UR0875, INRA Toulouse, 31326 Castanet-Tolosan, France
Ecolab, Université de Toulouse, CNRS, INPT, UPS, 31400 Toulouse, France
CNRS, UMR EcoFoG, AgroParisTech, Cirad, INRA, Université des Antilles, Université de Guyane, 97310 Kourou, France
Author to whom correspondence should be addressed.
Toxins 2019, 11(12), 732;
Received: 24 October 2019 / Revised: 6 December 2019 / Accepted: 11 December 2019 / Published: 14 December 2019
(This article belongs to the Special Issue Venom Proteomics and Transcriptomics)
In the mutualisms involving certain pseudomyrmicine ants and different myrmecophytes (i.e., plants sheltering colonies of specialized “plant-ant” species in hollow structures), the ant venom contributes to the host plant biotic defenses by inducing the rapid paralysis of defoliating insects and causing intense pain to browsing mammals. Using integrated transcriptomic and proteomic approaches, we identified the venom peptidome of the plant-ant Tetraponera aethiops (Pseudomyrmecinae). The transcriptomic analysis of its venom glands revealed that 40% of the expressed contigs encoded only seven peptide precursors related to the ant venom peptides from the A-superfamily. Among the 12 peptide masses detected by liquid chromatography-mass spectrometry (LC–MS), nine mature peptide sequences were characterized and confirmed through proteomic analysis. These venom peptides, called pseudomyrmecitoxins (PSDTX), share amino acid sequence identities with myrmeciitoxins known for their dual offensive and defensive functions on both insects and mammals. Furthermore, we demonstrated through reduction/alkylation of the crude venom that four PSDTXs were homo- and heterodimeric. Thus, we provide the first insights into the defensive venom composition of the ant genus Tetraponera indicative of a streamlined peptidome. View Full-Text
Keywords: defensive venom; dimeric peptides; peptidome; Tetraponera aethiops defensive venom; dimeric peptides; peptidome; Tetraponera aethiops
Show Figures

Graphical abstract

MDPI and ACS Style

Barassé, V.; Touchard, A.; Téné, N.; Tindo, M.; Kenne, M.; Klopp, C.; Dejean, A.; Bonnafé, E.; Treilhou, M. The Peptide Venom Composition of the Fierce Stinging Ant Tetraponera aethiops (Formicidae: Pseudomyrmecinae). Toxins 2019, 11, 732.

AMA Style

Barassé V, Touchard A, Téné N, Tindo M, Kenne M, Klopp C, Dejean A, Bonnafé E, Treilhou M. The Peptide Venom Composition of the Fierce Stinging Ant Tetraponera aethiops (Formicidae: Pseudomyrmecinae). Toxins. 2019; 11(12):732.

Chicago/Turabian Style

Barassé, Valentine, Axel Touchard, Nathan Téné, Maurice Tindo, Martin Kenne, Christophe Klopp, Alain Dejean, Elsa Bonnafé, and Michel Treilhou. 2019. "The Peptide Venom Composition of the Fierce Stinging Ant Tetraponera aethiops (Formicidae: Pseudomyrmecinae)" Toxins 11, no. 12: 732.

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop