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Toxins 2018, 10(7), 290; https://doi.org/10.3390/toxins10070290

The Effect of Cyanobacterial LPS Antagonist (CyP) on Cytokines and Micro-RNA Expression Induced by Porphyromonas gingivalis LPS

Laboratorio di Biologia Applicata, Dipartimento di Medicina e Chirurgia, Università degli Studi dell’Insubria, Via Dunant, 3-21100 Varese, Italy
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Received: 14 June 2018 / Revised: 6 July 2018 / Accepted: 11 July 2018 / Published: 16 July 2018
(This article belongs to the Special Issue Lipopolysaccharide: Bacterial Endotoxin)
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Abstract

Lipopolysaccharide (LPS) from Porphyromonas gingivalis (Pg-LPS) is a key bacterial structure involved in the maintenance of a chronic pro-inflammatory environment during periodontitis. Similar to other gram-negative LPS, Pg-LPS induces the release of pro-inflammatory cytokines through interaction with Toll-Like Receptor 4 (TLR4) and is able to stimulate negative TLR4 regulatory pathways, such as those involving microRNA (miRNA). In this work, we employed CyP, an LPS with TLR4-MD2 antagonist activity obtained from the cyanobacterium Oscillatoria planktothrix FP1, to study the effects on pro-inflammatory cytokine production and miRNA expression in human monocytic THP-1 cells stimulated with Pg-LPS or E. coli LPS (Ec-LPS). Results showed that CyP inhibited TNF-α, IL-1β and IL-8 expression more efficiently when co-incubated with Pg-LPS rather than with Ec-LPS. The inhibition of pro-inflammatory cytokine production was maintained even when CyP was added 2 h after LPS. The analysis of the effects of CyP on miRNA expression showed that, although being an antagonist, CyP did not inhibit miR-146a induced by Pg-LPS or Ec-LPS, whereas it significantly inhibited miR-155 only in the cultures stimulated with Ec-LPS. These results suggest that CyP may modulate the pro-inflammatory response induced by Pg-LPS, not only by blocking TLR4-MD2 complex, but also by preserving miR-146a expression. View Full-Text
Keywords: LPS; Porphyromonas gingivalis; Oscillatoria planktothrix FP1; TNF-α; IL-1β; IL-8; miRNA LPS; Porphyromonas gingivalis; Oscillatoria planktothrix FP1; TNF-α; IL-1β; IL-8; miRNA
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Molteni, M.; Bosi, A.; Rossetti, C. The Effect of Cyanobacterial LPS Antagonist (CyP) on Cytokines and Micro-RNA Expression Induced by Porphyromonas gingivalis LPS. Toxins 2018, 10, 290.

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