The Effect of Cyanobacterial LPS Antagonist (CyP) on Cytokines and Micro-RNA Expression Induced by Porphyromonas gingivalis LPS
Abstract
:1. Introduction
2. Results
2.1. CyP Dose-Dependently Inhibits Pro-Inflammatory Cytokine Production Induced by Pg-LPS
2.2. CyP Inhibits Pro-Inflammatory Cytokine Production Induced by Pg-LPS also When Added Two Hours after LPS
2.3. CyP Differently Affects miRNA Expression in THP-1 Cells Stimulated with Pg-LPS and Ec-LPS
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Cells and Reagents
5.2. THP-1 Cell Cultures
5.3. Cytokine ELISA
5.4. RNA Isolation
5.5. Cytokine Quantitative Real-Time PCR
5.6. MiRNA Quantitative Real-Time PCR
5.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Molteni, M.; Bosi, A.; Rossetti, C. The Effect of Cyanobacterial LPS Antagonist (CyP) on Cytokines and Micro-RNA Expression Induced by Porphyromonas gingivalis LPS. Toxins 2018, 10, 290. https://doi.org/10.3390/toxins10070290
Molteni M, Bosi A, Rossetti C. The Effect of Cyanobacterial LPS Antagonist (CyP) on Cytokines and Micro-RNA Expression Induced by Porphyromonas gingivalis LPS. Toxins. 2018; 10(7):290. https://doi.org/10.3390/toxins10070290
Chicago/Turabian StyleMolteni, Monica, Annalisa Bosi, and Carlo Rossetti. 2018. "The Effect of Cyanobacterial LPS Antagonist (CyP) on Cytokines and Micro-RNA Expression Induced by Porphyromonas gingivalis LPS" Toxins 10, no. 7: 290. https://doi.org/10.3390/toxins10070290
APA StyleMolteni, M., Bosi, A., & Rossetti, C. (2018). The Effect of Cyanobacterial LPS Antagonist (CyP) on Cytokines and Micro-RNA Expression Induced by Porphyromonas gingivalis LPS. Toxins, 10(7), 290. https://doi.org/10.3390/toxins10070290