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Toxins 2018, 10(3), 119; https://doi.org/10.3390/toxins10030119

An Imaging Surface Plasmon Resonance Biosensor Assay for the Detection of T-2 Toxin and Masked T-2 Toxin-3-Glucoside in Wheat

Mycotoxin Prevention and Applied Microbiology Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Peoria, IL 61604, USA
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Received: 25 January 2018 / Revised: 2 March 2018 / Accepted: 7 March 2018 / Published: 10 March 2018
(This article belongs to the Special Issue Advanced Sensors for Toxins)
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Abstract

A sensitive, rapid, and reproducible imaging surface plasmon resonance (iSPR) biosensor assay was developed to detect T-2 toxin and T-2 toxin-3-glucoside (T2-G) in wheat. In this competitive assay, an amplification strategy was used after conjugating a secondary antibody (Ab2) with gold nanoparticles. Wheat samples were extracted with a methanol/water mixture (80:20 v/v), then diluted with an equal volume of primary antibody (Ab1) for analysis. Matrix-matched calibration curves were prepared to determine T-2 toxin and T2-G. Recovery studies were conducted at three spiking levels in blank wheat. Mean recoveries ranged from 86 to 90%, with relative standard deviations for repeatability (RSDr) of less than 6%. Limits of detection were 1.2 ng/mL of T-2 toxin and 0.9 ng/mL of T2-G, equivalent to their levels in wheat, of 48 and 36 µg/kg, respectively. The developed iSPR assay was rapid and provided enough sensitivity for the monitoring of T-2 toxin/T2-G in wheat. This is the first iSPR assay useful for detecting the “masked” T2-G in wheat. View Full-Text
Keywords: biosensor; iSPR; gold nanoparticle; T-2 toxin; T-2 toxin-3-glucoside; wheat biosensor; iSPR; gold nanoparticle; T-2 toxin; T-2 toxin-3-glucoside; wheat
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Hossain, M.Z.; McCormick, S.P.; Maragos, C.M. An Imaging Surface Plasmon Resonance Biosensor Assay for the Detection of T-2 Toxin and Masked T-2 Toxin-3-Glucoside in Wheat. Toxins 2018, 10, 119.

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