Next Article in Journal
Tectus niloticus (Tegulidae, Gastropod) as a Novel Vector of Ciguatera Poisoning: Clinical Characterization and Follow-Up of a Mass Poisoning Event in Nuku Hiva Island (French Polynesia)
Next Article in Special Issue
An Imaging Surface Plasmon Resonance Biosensor Assay for the Detection of T-2 Toxin and Masked T-2 Toxin-3-Glucoside in Wheat
Previous Article in Journal
Anti-Inflammatory and Immune Regulatory Actions of Naja naja atra Venom
Previous Article in Special Issue
An On-Site Simultaneous Semi-Quantification of Aflatoxin B1, Zearalenone, and T-2 Toxin in Maize- and Cereal-Based Feed via Multicolor Immunochromatographic Assay
Open AccessArticle

A Rapid Label-Free Fluorescent Aptasensor PicoGreen-Based Strategy for Aflatoxin B1 Detection in Traditional Chinese Medicines

Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China
School of Pharmacy JiangSu University, Zhenjiang 212013, China
National Research Council of Italy, CNR-ISPA, Via G. Amendola, 122/O, I-70126 Bari, Italy
Author to whom correspondence should be addressed.
Toxins 2018, 10(3), 101;
Received: 8 January 2018 / Revised: 19 February 2018 / Accepted: 26 February 2018 / Published: 28 February 2018
(This article belongs to the Special Issue Advanced Sensors for Toxins)
Aflatoxin B1 (AFB1) is a very hazardous carcinogen, readily contaminating foodstuffs and traditional Chinese medicines (TCMs) that has inspired increasing health concerns due to dietary exposure. Colloidal nanocrystals have been proposed as optical labels for aptasensor assembly, but these typically require tedious multistep conjugation and suffer from unsatisfactory robustness when used for complex matrices. In the present study, we report a rapid and sensitive method for screening for trace AFB1 levels in TCMs using a label-free fluorescent aptasensor PicoGreen dye-based strategy. Using PicoGreen to selectively measure complementary double-stranded DNA, fluorescence enhancement due to dsDNA is ‘turned off’ in the presence of AFB1 due binding of aptamer target over complementary sequence. Self-assembly of a label-free fluorescent aptasensor based on AFB1 aptamer and PicoGreen dye was performed. Due to competition between the complementary sequence and AFB1 target, this rapid method was capable of highly sensitive and selective screening for AFB1 in five types of TCMs. This proposed approach had a limit of detection as low as 0.1 μg·L−1 and good linearity with a range of 0.1–10 μg·L−1 (0.1–10 ppb). Among the 20 samples tested, 6 batches were found to be contaminated with AFB1 using this method, which was confirmed using sophisticated liquid chromatography-electrospray ionization-tandem mass spectrometry/mass spectrometry analysis. The results of this study indicate the developed method has the potential to be a simple, quick, and sensitive tool for detecting AFB1 in TCMs. View Full-Text
Keywords: aflatoxin B1; aptamer; PicoGreen; fluorescence; traditional Chinese medicines aflatoxin B1; aptamer; PicoGreen; fluorescence; traditional Chinese medicines
Show Figures

Figure 1

MDPI and ACS Style

Zhang, C.; Dou, X.; Zhang, L.; Sun, M.; Zhao, M.; OuYang, Z.; Kong, D.; Antonio, F.L.; Yang, M. A Rapid Label-Free Fluorescent Aptasensor PicoGreen-Based Strategy for Aflatoxin B1 Detection in Traditional Chinese Medicines. Toxins 2018, 10, 101.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop