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Toxins 2018, 10(2), 74; https://doi.org/10.3390/toxins10020074

Bacillus subtilis Type I antitoxin SR6 Promotes Degradation of Toxin yonT mRNA and Is Required to Prevent Toxic yoyJ Overexpression

Friedrich-Schiller-Universität Jena, Fakultät für Biowissenschaften, Matthias-Schleiden-Institut, AG Bakteriengenetik, Philosophenweg 12, D-07743 Jena, Germany
Current address: Laboratorium für Mikrobiologie, Fachbereich Chemie, Philipps-Universität Marburg, Hans-Meerwein-Straße 4, D-35043 Marburg, Germany.
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Received: 18 January 2018 / Revised: 30 January 2018 / Accepted: 4 February 2018 / Published: 7 February 2018
(This article belongs to the Special Issue Toxin-antitoxin (TA) systems)
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Abstract

yonT/SR6 is the second type I toxin-antitoxin (TA) system encoded on prophage SPβ in the B. subtilis chromosome. The yonT ORF specifying a 58 aa toxin is transcribed on a polycistronic mRNA under control of the yonT promoter. The antitoxin SR6 is a 100 nt antisense RNA that overlaps yonT at its 3′ end and the downstream gene yoyJ encoding a second, much weaker, toxin at its 5′ end. SR6 displays a half-life of >60 min, whereas yonT mRNA is less stable with a half-life of ≈8 min. SR6 is in significant excess over yonT mRNA except in minimal medium with glucose. It interacts with the 3′ UTR of yonT mRNA, thereby promoting its degradation by RNase III. By contrast, SR6 does not affect the amount or half-life of yoyJ mRNA. However, in its absence, a yoyJ overexpression plasmid could not be established in Bacillus subtilis suggesting that SR6 inhibits yoyJ translation by directly binding to its ribosome-binding site. While the amounts of both yonT RNA and SR6 were affected by vancomycin, manganese, heat-shock and ethanol stress as well as iron limitation, oxygen stress decreased only the amount of SR6. View Full-Text
Keywords: yonT/SR6; sRNA; small regulatory RNA; Bacillus subtilis; type I toxin-antitoxin system; multistress responsive TA system yonT/SR6; sRNA; small regulatory RNA; Bacillus subtilis; type I toxin-antitoxin system; multistress responsive TA system
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Reif, C.; Löser, C.; Brantl, S. Bacillus subtilis Type I antitoxin SR6 Promotes Degradation of Toxin yonT mRNA and Is Required to Prevent Toxic yoyJ Overexpression. Toxins 2018, 10, 74.

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