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Open AccessArticle

Aspergillus flavus NRRL 3251 Growth, Oxidative Status, and Aflatoxins Production Ability In Vitro under Different Illumination Regimes

1
Department of Applied Chemistry and Ecology, Faculty of Food Technology, Josip Juraj Strossmayer University of Osijek, Franje Kuhača 20, Osijek 31000, Croatia
2
Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences Vienna (BOKU), Konrad Lorenzstr. 20, Tulln 3430, Austria
3
Department of Food technology, University North, Trg dr. Žarka Dolinara 1, Koprivnica 48000, Croatia
4
Inspecto Ltd., Industrijska zona Nemetin, Vukovarska cesta 239b, Osijek 31000, Croatia
5
Department of Microbiology, Babcock University, Ilishan Remo 121103, Ogun State, Nigeria
6
Institute for Global Food Security, School of Biological Sciences, Queen’s University Belfast, University Road, Belfast BT7 1NN, Northern Ireland, UK
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Toxins 2018, 10(12), 528; https://doi.org/10.3390/toxins10120528
Received: 5 November 2018 / Revised: 5 December 2018 / Accepted: 6 December 2018 / Published: 10 December 2018
(This article belongs to the Collection Aflatoxins)
Aspergillus flavus is the most important mycotoxin-producing fungus involved in the global episodes of aflatoxin B1 contamination of crops at both the pre-harvest and post-harvest stages. However, in order to effectively control aflatoxin contamination in crops using antiaflatoxigenic and/or antifungal compounds, some of which are photosensitive, a proper understanding of the photo-sensitive physiology of potential experimental strains need to be documented. The purpose of the study is therefore to evaluate the effect of visible (VIS) light illumination on growth and conidiation, aflatoxin production ability and modulation of A. flavus oxidative status during in vitro experiment. Aflatoxigenic A. flavus strain was inoculated in aflatoxin-inducing YES media and incubated under three different VIS illumination regimes during a 168 h growth period at 29 °C. VIS illumination reduced A. flavus mycelia biomass yield, both during growth on plates and in liquid media, promoted conidiation and increased the aflatoxin production. Furthermore, aflatoxin production increased with increased reactive oxidative species (ROS) levels at 96 h of growth, confirming illumination-driven oxidative stress modulation activity on A. flavus cells. View Full-Text
Keywords: VIS light illumination; Aspergillus flavus; mycotoxins; mycelial growth; oxidative status modulation; aflatoxin production; optogenetics VIS light illumination; Aspergillus flavus; mycotoxins; mycelial growth; oxidative status modulation; aflatoxin production; optogenetics
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Kovač, T.; Šarkanj, B.; Crevar, B.; Kovač, M.; Lončarić, A.; Strelec, I.; Ezekiel, C.N.; Sulyok, M.; Krska, R. Aspergillus flavus NRRL 3251 Growth, Oxidative Status, and Aflatoxins Production Ability In Vitro under Different Illumination Regimes. Toxins 2018, 10, 528.

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