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Toxins 2018, 10(11), 460; https://doi.org/10.3390/toxins10110460

Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia

1
Institute for Animal Husbandry, Autoput 16, 11080 Belgrade-Zemun, Serbia
2
Maize Research Institute “Zemun Polje”, Slobodana Bajića 1, 11185 Belgrade, Serbia
3
Institute of Food Technology and Biochemistry, Faculty of Agriculture, University of Belgrade, Nemanjina 16, 11080 Belgrade-Zemun, Serbia
4
Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia
*
Author to whom correspondence should be addressed.
Received: 18 October 2018 / Revised: 2 November 2018 / Accepted: 5 November 2018 / Published: 8 November 2018
(This article belongs to the Special Issue Recent Advances in Fusarium Research)
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Abstract

Fusarium graminearum as the main causal agent of Fusarium head blight (FHB) and its ability to produce trichothecenes was investigated by molecular techniques. A total of 37 strains isolated from the wheat, harvested in Serbia in 2005, 2008 and 2015, and previously designated by morphological observation as F. graminearum, were used for trichothecene genotypes characterization. The strains were identified using the species-specific primer set FG16R/FG16F while genotypic characterization was done using specific TRI13 and TRI3 sequences of the trichothecene gene clusters. The PCR assays identified all strains as species of F. graminearum sensu stricto with the DON/15-ADON genotype. The quantification of the mycotoxin (DON) was performed using the biochemical assay. The high levels of DON (>20,000 µg kg−1) were recorded in all of the strains from 2005, four strains from 2008 and two strains from 2015. Weather data of the investigated seasons, showed that the optimal temperature, frequent rains and high relative humidity (RH) was very favourable for the development of F. graminearum, affecting the DON biosynthesis. View Full-Text
Keywords: winter wheat; Fusarium head blight; Fusarium graminearum; trichothecene genotypes winter wheat; Fusarium head blight; Fusarium graminearum; trichothecene genotypes
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Krnjaja, V.; Stanković, S.; Obradović, A.; Petrović, T.; Mandić, V.; Bijelić, Z.; Božić, M. Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia. Toxins 2018, 10, 460.

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