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Toxins 2018, 10(11), 455; https://doi.org/10.3390/toxins10110455

Panton-Valentine Leucocidin Proves Direct Neuronal Targeting and Its Early Neuronal and Glial Impacts a Rabbit Retinal Explant Model

1
Université de Strasbourg, Hôpitaux Universitaires de Strasbourg, Fédération de Médecine Translationnelle de Strasbourg, EA7290 Virulence Bactérienne Précoce, Institut de Bactériologie, F-67000 Strasbourg, France
2
Department of Translational Medicine and Neurogenetics, Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS UMR_7104, Inserm U 964, Université de Strasbourg, F-67404 Illkirch, France
3
Sorbonne Université, INSERM, CNRS, Institut de la Vision, 17 rue Moreau, 75012 Paris, France
4
Hôpitaux Universitaires de Strasbourg, Service d’Ophtalmologie du Nouvel Hôpital Civil, F-67000 Strasbourg, France
*
Author to whom correspondence should be addressed.
Received: 13 September 2018 / Revised: 15 October 2018 / Accepted: 30 October 2018 / Published: 4 November 2018
(This article belongs to the Special Issue Leukotoxins)
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Abstract

Panton-Valentine leukocidin (PVL) retinal intoxication induces glial activation and inflammatory response via the interaction with retinal neurons. In this study, rabbit retinal explant was used as a model to study neuronal and glial consequences of PVL intoxication. Retinal explants were treated with different concentrations of PVL. PVL location and neuronal and glial changes were examined using immunohistochemistry. Some inflammatory factors were quantified using RT-qPCR at 4 and 8 h. These results were compared with those of control explants. PVL co-localized rapidly with retinal ganglion cells and with horizontal cells. PVL induced Müller and microglial cell activation. Retinal structure was altered and some amacrine and microglial cells underwent apoptosis. Glial activation and cell apoptosis increased in a PVL concentration- and time-dependent manner. IL-6 and IL-8 expression increased in PVL-treated explants but less than in control explants, which may indicate that other factors were responsible for glial activation and retinal apoptosis. On retinal explants, PVL co-localized with neuronal cells and induced glial activation together with microglial apoptosis, which confirms previous results observed in in vivo model. Rabbit retinal explant seems to be suitable model to further study the process of PVL leading to glial activation and retinal cells apoptosis. View Full-Text
Keywords: Panton-Valentine leukocidin; apoptosis; Müller cell activation; microglial cell activation; retinal explant Panton-Valentine leukocidin; apoptosis; Müller cell activation; microglial cell activation; retinal explant
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Liu, X.; Roux, M.J.; Picaud, S.; Keller, D.; Sauer, A.; Heitz, P.; Prévost, G.; Gaucher, D. Panton-Valentine Leucocidin Proves Direct Neuronal Targeting and Its Early Neuronal and Glial Impacts a Rabbit Retinal Explant Model. Toxins 2018, 10, 455.

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