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Article

Bee Venom Suppresses the Differentiation of Preadipocytes and High Fat Diet-Induced Obesity by Inhibiting Adipogenesis

1
Department of Pharmacology, College of Korean Medicine, Sang-ji University, Wonju-si, Gangwon-do 26339, Korea
2
Catholic Precision Medicine Research Center, College of Medicine, The Catholic University of Korea, 222, Banpo-daero, Seocho-gu, Seoul 06591, Korea
3
Department of Herbology, College of Korean Medicine, Daegu Hanny University, Suseong-gu, Deagu 42158, Korea
4
Department of Rehabilitation Medicine of Korean Medicine and Neuropsychiatry, College of Korean Medicine, Sang-ji University, Wonju-si, Gangwon-do 26339, Korea
*
Author to whom correspondence should be addressed.
Received: 8 December 2017 / Revised: 21 December 2017 / Accepted: 21 December 2017 / Published: 24 December 2017
(This article belongs to the Special Issue Toxins in Drug Discovery and Pharmacology)
Bee venom (BV) has been widely used in the treatment of certain immune-related diseases. It has been used for pain relief and in the treatment of chronic inflammatory diseases. Despite its extensive use, there is little documented evidence to demonstrate its medicinal utility against obesity. In this study, we demonstrated the inhibitory effects of BV on adipocyte differentiation in 3T3-L1 cells and on a high fat diet (HFD)-induced obesity mouse model through the inhibition of adipogenesis. BV inhibited lipid accumulation, visualized by Oil Red O staining, without cytotoxicity in the 3T3-L1 cells. Male C57BL/6 mice were fed either a HFD or a control diet for 8 weeks, and BV (0.1 mg/kg or 1 mg/kg) or saline was injected during the last 4 weeks. BV-treated mice showed a reduced body weight gain. BV was shown to inhibit adipogenesis by downregulating the expression of the transcription factors CCAAT/enhancer-binding proteins (C/EBPs) and the peroxisome proliferator-activated receptor gamma (PPARγ), using RT-qPCR and Western blotting. BV induced the phosphorylation of AMP-activated kinase (AMPK) and acetyl-CoA carboxylase (ACC) in the cell line and in obese mice. These findings demonstrate that BV mediates anti-obesity/differentiation effects by suppressing obesity-related transcription factors. View Full-Text
Keywords: Bee venom; PPARγ; AMPK; MAPK; adipogenesis Bee venom; PPARγ; AMPK; MAPK; adipogenesis
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MDPI and ACS Style

Cheon, S.-Y.; Chung, K.-S.; Roh, S.-S.; Cha, Y.-Y.; An, H.-J. Bee Venom Suppresses the Differentiation of Preadipocytes and High Fat Diet-Induced Obesity by Inhibiting Adipogenesis. Toxins 2018, 10, 9. https://doi.org/10.3390/toxins10010009

AMA Style

Cheon S-Y, Chung K-S, Roh S-S, Cha Y-Y, An H-J. Bee Venom Suppresses the Differentiation of Preadipocytes and High Fat Diet-Induced Obesity by Inhibiting Adipogenesis. Toxins. 2018; 10(1):9. https://doi.org/10.3390/toxins10010009

Chicago/Turabian Style

Cheon, Se-Yun, Kyung-Sook Chung, Seong-Soo Roh, Yun-Yeop Cha, and Hyo-Jin An. 2018. "Bee Venom Suppresses the Differentiation of Preadipocytes and High Fat Diet-Induced Obesity by Inhibiting Adipogenesis" Toxins 10, no. 1: 9. https://doi.org/10.3390/toxins10010009

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