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Open AccessArticle

Are miRNA-103, miRNA-107 and miRNA-122 Involved in the Prevention of Liver Steatosis Induced by Resveratrol?

Nutrition and Obesity Group, Department of Nutrition and Food Science, University of the Basque Country (UPV/EHU) and Lucio Lascaray Research Institute, Vitoria 01006, Spain
CIBERobn Physiopathology of Obesity and Nutrition, Institute of Health Carlos III, Madrid 28029, Spain
Nutrition and Food Science, Faculty of Biochemistry and Biological Sciences, National University of Litoral, Santa Fe 3000, Argentina
Author to whom correspondence should be addressed.
Nutrients 2017, 9(4), 360;
Received: 25 February 2017 / Revised: 31 March 2017 / Accepted: 1 April 2017 / Published: 4 April 2017
(This article belongs to the Special Issue Precision Nutrition and Metabolic Syndrome Management)
The aim of the present study was to determine whether the reduction in liver fat previously observed in our laboratory in a cohort of rats which had been fed an obesogenic diet was mediated by changes in the expression of microRNA (miRNA)-103-3p, miRNA-107-3p and miRNA-122-5p, which represent 70% of total miRNAs in the liver, as well as in their target genes. The expression of the three analysed miRNAs was reduced in rats treated with resveratrol. A reduction in sterol-regulatory element binding protein 1 (SREBP1) and an increase in carnitine palmitoyltransferase 1a (CPT1a) were observed in resveratrol-treated rats. No changes were found in fatty acid synthase (FAS). In cultured hepatocytes, SREBP1 protein was increased after the transfection of each miRNA. FAS protein expression was decreased after the transfection of miRNA-122-5p, and CPT1a protein was down-regulated by the over-expression of miRNA-107-3p. This study provides new evidences which show that srebf1 is a target gene for miRNA-103-3p and miRNA-107-3p, fasn a target gene for miRNA-122-5p and cpt1a a target gene for miRNA-107-3p. Moreover, the reduction in liver steatosis induced by resveratrol in rats fed an obesegenic diet is mediated, at least in part, by the increase in CPT1a protein expression and activity, via a decrease in miRNA-107-3p expression. View Full-Text
Keywords: miRNA-103; miRNA-107; miRNA-122; steatosis; liver; resveratrol; rat miRNA-103; miRNA-107; miRNA-122; steatosis; liver; resveratrol; rat
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Gracia, A.; Fernández-Quintela, A.; Miranda, J.; Eseberri, I.; González, M.; Portillo, M.P. Are miRNA-103, miRNA-107 and miRNA-122 Involved in the Prevention of Liver Steatosis Induced by Resveratrol? Nutrients 2017, 9, 360.

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