Live Faecalibacterium prausnitzii Does Not Enhance Epithelial Barrier Integrity in an Apical Anaerobic Co-Culture Model of the Large Intestine
Abstract
:1. Introduction
2. Materials and Methods
2.1. F. prausnitzii Culturing Conditions
2.2. Caco-2 Cell Culturing Conditions
2.3. Apical Anaerobic Co-Culture Model
2.4. TEER Experiment Protocol
2.5. Survival of F. prausnitzii in Anaerobic Cell Culture Media
2.6. TEER and Viability of Caco-2 Cells Using a Combination of Cell and Bacterial Culture Media
2.7. Viability of the F. prausnitzii Strains in the Apical Anaerobic Co-Culture Model Using a Combination of Cell and Bacterial Culture Media
2.8. TEER Assay Using F. prausnitzii in Different Apical Media
2.9. TEER Assay Using Live and UV-Killed F. prausnitzii
2.10. Statistical Analysis
3. Results and Discussion
3.1. F. prausnitzii Did Not Grow in an Anaerobic Cell Culture Medium
3.2. Bacterial Medium Did Not Affect Caco-2 Viability, but Reduced TEER at High Concentrations
3.3. Two F. prausnitzii Strains Grew in 50% Bacterial Medium When Co-Cultured with Caco-2 Cells
3.4. Live F. prausnitzii Did Not Alter TEER across Caco-2 Cells
3.5. UV-Killed F. prausnitzii Decreased TEER
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Maier, E.; Anderson, R.C.; Roy, N.C. Live Faecalibacterium prausnitzii Does Not Enhance Epithelial Barrier Integrity in an Apical Anaerobic Co-Culture Model of the Large Intestine. Nutrients 2017, 9, 1349. https://doi.org/10.3390/nu9121349
Maier E, Anderson RC, Roy NC. Live Faecalibacterium prausnitzii Does Not Enhance Epithelial Barrier Integrity in an Apical Anaerobic Co-Culture Model of the Large Intestine. Nutrients. 2017; 9(12):1349. https://doi.org/10.3390/nu9121349
Chicago/Turabian StyleMaier, Eva, Rachel C. Anderson, and Nicole C. Roy. 2017. "Live Faecalibacterium prausnitzii Does Not Enhance Epithelial Barrier Integrity in an Apical Anaerobic Co-Culture Model of the Large Intestine" Nutrients 9, no. 12: 1349. https://doi.org/10.3390/nu9121349