Recovery Effects of Oral Administration of Glucosylceramide and Beet Extract on Skin Barrier Destruction by UVB in Hairless Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Animals
2.3. Test Sample and Schedule
2.4. Measurement of Water Contents of Stratum Corneum, and TEWL
2.5. Collection of Mouse Stratum Corneum
2.6. Extraction Method and Lipid Analysis by HPTLC
2.7. Measurement of Stratum Corneum Mass
2.8. Hematoxylin and Eosin Staining
2.9. Measurement of Epidermis Thickness
2.10. Data and Statistical Analysis
3. Results
3.1. Body Weight Change after Oral Administration of Beet GlcCer or Beet Extract in UVB-Irradiated Mice
3.2. Epidermal Hyperplasia Following UVB Irradiation
3.3. Comparison of Ceramide Contents in Stratum Corneum among Mouse Groups
3.4. TEWL and Water Contents in Stratum Corneum
4. Discussion
5. Conclusions
Author Contributions
Conflicts of Interest
References
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Group | UVB Irradiation (mJ/cm2) | Composition of Test Sample (mg/Mouse/Day) | ||
---|---|---|---|---|
GlcCer | Beet Ethanol Extract | Processed Starch | ||
Normal | None | 0 | 0 | 22 |
Control | 200 | 0 | 0 | 22 |
Beet GlcCer | 200 | 0.3 | 0 | 22 |
Beet Extract | 200 | (0.3) 1 | 8.0 | 22 |
Events/Day | −7 d | −6 d | −5 d | −4 d | −3 d | −2 d | −1 d | 0 d | 1 d | 2 d | 3 d | 4 d | 5 d | 6 d | 7 d |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sample administration | ◯ | ◯ | ◯ | ◯ | ◯ | ◯ | ◯ | ◯ | ◯ | ◯ | ◯ | ◯ | ◯ | ◯ | |
UV irradiation | ◯ | ||||||||||||||
Body weight | ◯ | ◯ | ◯ | ◯ | ◯ | ◯ | ◯ | ◯ | ◯ | ◯ | ◯ | ◯ | |||
Water content, TEWL | ◯ | ◯ | ◯ | ||||||||||||
Ceramide content | ◯ | ||||||||||||||
HE staining | ◯ |
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Tokudome, Y.; Masutani, N.; Uchino, S.; Fukai, H. Recovery Effects of Oral Administration of Glucosylceramide and Beet Extract on Skin Barrier Destruction by UVB in Hairless Mice. Nutrients 2017, 9, 1178. https://doi.org/10.3390/nu9111178
Tokudome Y, Masutani N, Uchino S, Fukai H. Recovery Effects of Oral Administration of Glucosylceramide and Beet Extract on Skin Barrier Destruction by UVB in Hairless Mice. Nutrients. 2017; 9(11):1178. https://doi.org/10.3390/nu9111178
Chicago/Turabian StyleTokudome, Yoshihiro, Noriomi Masutani, Shohei Uchino, and Hisano Fukai. 2017. "Recovery Effects of Oral Administration of Glucosylceramide and Beet Extract on Skin Barrier Destruction by UVB in Hairless Mice" Nutrients 9, no. 11: 1178. https://doi.org/10.3390/nu9111178
APA StyleTokudome, Y., Masutani, N., Uchino, S., & Fukai, H. (2017). Recovery Effects of Oral Administration of Glucosylceramide and Beet Extract on Skin Barrier Destruction by UVB in Hairless Mice. Nutrients, 9(11), 1178. https://doi.org/10.3390/nu9111178